Transfection of a DNA locus that mediates the conversion of 10T1 2 fibroblasts to myoblasts

  title={Transfection of a DNA locus that mediates the conversion of 
 fibroblasts to myoblasts},
  author={Andrew B. Lassar and Bruce M. Paterson and Harold Weintraub},

Commitment of mouse fibroblasts to adipocyte differentiation by DNA transfection.

Transfection of a trans-regulatory gene (or genes) from 3T3-F442A or human fat cells into 3T2-C2 cells is sufficient to commit 3T1-C1 cells to adipocyte differentiation.

Effect of cellular determination on oncogenic transformation by chemicals and oncogenes

The presence of as many as six copies of the c-Ha-ras gene per genome did not prevent the formation of striated muscle cells which expressed immunologically detectable muscle-specific myosin and the expression of the determination gene for myogenesis or prevent end-stage myogenic differentiation.

Effect of myogenic determination on tumorigenicity of chemically transformed 10T1/2 cells

Induction of myogenic determination was not sufficient to abolish the tumorigenic phenotype of MCA CI 15C1 cells, but several tumors showed decreased levels of MyoD1 mRNA, suggesting that growth in vivo either selected for or caused decreased determination gene expression.

- 5-5 ’ Azacytidine Enhances Exogenous Gene Expression in Skeletal Muscle

5’ azacytidine enhances the level and the time span of exogenous gene expression in mu scle cells facilitating gene therapy studies, as shown by DNA restriction analysis.



DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.

The feasibility of transforming mouse cells deficient in adenine phosphoribosyltransferase to the aprt+ phenotype by means of DNA-mediated gene transfer is demonstrated.

Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.

A bacterial gene conferring resistance to neomycin-kanamycin antibiotics has been inserted into SV40 hybrid plasmid vectors and introduced into cultured mammalian cells by DNA transfusion and it is shown that cell transformation to G418 resistance is an efficient means for cotransformation of nonselected genes.

Inactive X chromosome DNA does not function in DNA-mediated cell transformation for the hypoxanthine phosphoribosyltransferase gene.

  • R. LiskayR. Evans
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1980
There is a difference between the DNA, per se, of the active and inactive X at (or near) the HPRT locus and that this difference could account, at least in part, for its inactivation.

Expression and regulation of chicken actin genes introduced into mouse myogenic and nonmyogenic cells.

The results suggest that the tissue specificity of expression is maintained but the pattern of gene regulation for the sarcomeric actins is not, and the decrease in the levels of beta-actin RNA during C2 cell differentiation provides a model system in which to study gene repression during development.

Changes in phenotypic expression in embryonic and adult cells treated with 5‐azacytidine

5‐azacytidine induced the formation of biochemically differentiated myotubes, adipocytes, and chondrocytes in the mouse embryo cell line and the induction of the muscle phenotype was cell cycle specific, while the adipocyte phenotype is also induced maximally in cells treated during early S phase.

Differentiation, not determination, regulates muscle gene activation: transfection of troponin I genes into multipotential and muscle lineages of 10T1/2 cells

Myoblast lineage determination, therefore, does not appear to act directly on TnI and other muscle genes but likely establishes a regulatory control system that mediates expression of differentiation-specific transcription signals.

In vitro methylation of the hamster adenine phosphoribosyltransferase gene inhibits its expression in mouse L cells.

The results show that the expression of certain genes may be inhibited by site-specific methylation of these sequences and suggest that methylation may play a direct role in the regulation of gene expression.