• Chemistry
  • Published 2012

Transfection efficiencies of adenoviruses Ad5-GFP,Ad5F35-GFP and Lipofectamine~(TM) 2000 to four cell lines

@inproceedings{Zhiguang2012TransfectionEO,
  title={Transfection efficiencies of adenoviruses Ad5-GFP,Ad5F35-GFP and Lipofectamine~(TM) 2000 to four cell lines},
  author={Tu Zhi-guang},
  year={2012}
}
Objective To comparing the transfection efficiencies adenoviruses Ad5-GFP,Ad5F35-GFP and LipofectamineTM 2000 to four cell lines so as to obtain the suitable cell line for Ad5F35-GFP and the suitable transfection method for various cells.Methods Human chronic granulocytic leukemia K562 cells,human peripherhal blood mononuclear cells(PBMCs),mouse adipocyte 3T3-L1 strain and mouse liver cancer Hep A 1-6 cells were transfected with adenoviruses Ad5-GFP,Ad5F35-GFP and plasmid pEGFP-N1(with… CONTINUE READING