Transfected Rat cMOAT Is Functionally Expressed on the Apical Membrane in Madin-Darby Canine Kidney (MDCK) Cells

@article{Kinoshita2004TransfectedRC,
  title={Transfected Rat cMOAT Is Functionally Expressed on the Apical Membrane in Madin-Darby Canine Kidney (MDCK) Cells},
  author={Setsuo Kinoshita and H. Suzuki and K. Ito and K. Kume and T. Shimizu and Y. Sugiyama},
  journal={Pharmaceutical Research},
  year={2004},
  volume={15},
  pages={1851-1856}
}
  • Setsuo Kinoshita, H. Suzuki, +3 authors Y. Sugiyama
  • Published 2004
  • Biology, Medicine
  • Pharmaceutical Research
    • AbstractPurpose. The purpose of the present study is to investigate the expression of canalicular multispecific organic anion transporter (cMOAT) by its cDNA transfection in polarized Madin-Darby canine kidney cells (MDCK). Methods. MDCK cells were transfected with an expression vector (pCXN2) containing the rat cMOAT cDNA with lipofectamine to obtain the stable transfectant under G418. Cells from a single colony whose cMOAT expression was the highest were seeded to form a tight epithelial… CONTINUE READING
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