Transepithelial sulfate transport by avian renal proximal tubule epithelium in primary culture.

Abstract

The mechanisms and control of transepithelial inorganic sulfate (Si) transport by primary cultures of chick renal proximal tubule monolayers in Ussing chambers were determined. The competitive anion, S2 O 3 2- (5 mM), reduced both unidirectional reabsorptive and secretory fluxes and net Si reabsorption with no effect on electrophysiological properties. The carbonic anhydrase (CA) inhibitor ethoxzolamide decreased net Si reabsorption approximately 45%. CAII protein and activity were detected in isolated chick proximal tubules by immunoblots and biochemical assay, respectively. Cortisol reduced net Si reabsorption up to approximately 50% in a concentration-dependent manner. Thyroid hormone increased net Si reabsorption threefold in 24 h, and parathyroid hormone (PTH) acutely stimulated net Si reabsorption approximately 45%. These data indicate that CA participates in avian proximal tubule active transepithelial Si reabsorption, which cortisol directly inhibits and T3 and PTH directly stimulate.

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Cite this paper

@article{Dudas2002TransepithelialST, title={Transepithelial sulfate transport by avian renal proximal tubule epithelium in primary culture.}, author={Paul L Dudas and J. Larry Renfro}, journal={American journal of physiology. Regulatory, integrative and comparative physiology}, year={2002}, volume={283 6}, pages={R1354-61} }