Transduction peptides: from technology to physiology

@article{Joliot2004TransductionPF,
  title={Transduction peptides: from technology to physiology},
  author={Alain Joliot and Alain Prochiantz},
  journal={Nature Cell Biology},
  year={2004},
  volume={6},
  pages={189-196}
}
During the past fifteen years, a variety of peptides have been characterized for their ability to translocate into live cells. Most are efficient vectors that can internalize hydrophilic cargoes, and so provide a valuable biological (and potentially therapeutic) tool for targeting proteins into cells. Furthermore, translocation of cell-permeable peptides across the plasma membrane and their subsequent access to the cytosol, even when fused to large hydrophilic proteins, is challenging the… 

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References

SHOWING 1-10 OF 75 REFERENCES

A peptide carrier for the delivery of biologically active proteins into mammalian cells

A new strategy for protein delivery based on a short amphipathic peptide carrier, Pep-1, which is able to efficiently deliver a variety of peptides and proteins into several cell lines in a fully biologically active form, without the need for prior chemical covalent coupling or denaturation steps.

In vivo protein transduction: delivery of a biologically active protein into the mouse.

It is shown that intraperitoneal injection of the 120-kilodalton beta-galactosidase protein, fused to the protein transduction domain from the human immunodeficiency virus TAT protein, results in delivery of the biologically active fusion protein to all tissues in mice, including the brain.

Tilted peptides: a motif for membrane destabilization (Hypothesis)

This hypothesis overviews the discovery of tilted peptides, describes how they are detected and discusses how they could be involved in dynamic biological processes.

Messenger proteins: homeoproteins, TAT and others.

Cell membrane translocation of the N-terminal (1-28) part of the prion protein.

The design, synthesis, and evaluation of molecules that enable or enhance cellular uptake: peptoid molecular transporters.

Overall, a transporter has been developed that is superior to Tat(49-57), protease resistant, and more readily and economically prepared and suggest that the guanidinium groups of Tat( 49-57) play a greater role in facilitating cellular uptake than either charge or backbone structure.

Peptides fused to the amino-terminal end of diphtheria toxin are translocated to the cytosol

The results are compatible with a model in which the COOH-terminus of the A-fragment first crosses the membrane, whereas the NH2-terminal region follows behind, and neither hydrophobic nor highly charged sequences blocked translocation.
...