Transduction of cultured fish cells with recombinant baculoviruses.
@article{Leisy2003TransductionOC,
title={Transduction of cultured fish cells with recombinant baculoviruses.},
author={Douglas J. Leisy and Teresa D. Lewis and J. A. C. Leong and George F. Rohrmann},
journal={The Journal of general virology},
year={2003},
volume={84 Pt 5},
pages={
1173-8
}
}Five fish cell lines were tested for their ability to be transduced by Ac-CAlacZ, a recombinant baculovirus that is capable of expressing a beta-galactosidase reporter gene from the CAG promoter (consisting of a cytomegalovirus enhancer element, a chicken actin promoter and rabbit beta-globin termination sequences). TO (Tilapia ovary), EPC (carp), CHH-1 (Chum salmon heart fibroblast) and CHSE-214 (chinook salmon embryo) cells were transducible, as demonstrated by an in situ beta-galactosidase…
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References
SHOWING 1-10 OF 23 REFERENCES
Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector.
- Biology, EngineeringProceedings of the National Academy of Sciences of the United States of America
- 1999
Stably transduced derivatives have been selected from a substantial number of different cell types, suggesting that stable lines can be derived from any cell type that exhibits transient expression.
Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors.
- BiologyThe Journal of general virology
- 1997
The results suggest that the baculovirus vector is a good tool for gene delivery into various mammalian cells in order to study the function of foreign genes.
Efficient selection for high-expression transfectants with a novel eukaryotic vector.
- BiologyGene
- 1991
A Cell Line Derived from Tilapia Ovary
- Biology
- 1983
An epitheloid morphology cell line designated TO-2 was established from ovary of healthy adult tilapia hybrid using Leibovitz's L-15 medium with 10% fetal calf serum at 31°C and demonstrated to be free of bacterial, fungal and mycoplasma contaminations.
Efficient transduction of mammalian cells by a recombinant baculovirus having the vesicular stomatitis virus G glycoprotein.
- BiologyHuman gene therapy
- 1997
It is provided evidence that VSV G protein may enhance transduction by increasing the efficiency of escape of baculovirus from intracellular vesicles rather than by increasing cell binding or uptake of the virus.
Recombinant baculoviruses as mammalian cell gene-delivery vectors.
- Biology, EngineeringTrends in biotechnology
- 2002
Characterization of cell-surface determinants important for baculovirus infection.
- BiologyVirology
- 2001
Inhibition with purified lipids and susceptibility to the mutant CHO hamster cell lines deficient in phospholipids synthesis suggest that the interaction of gp64 and phospholIPids on the cell surface might play an important role in baculovirus infection into mammalian cells.
Baculovirus expression vectors : choice of expression vector.
- BiologyMethods in molecular biology
- 1992
Baculoviruses have been used for many years as effective pest-control agents; however, their interest to molecular virologists stems from their exploitation as helper-independent viral expression…
Genetic immunization of rainbow trout (Oncorhynchus mykiss) against infectious hematopoietic necrosis virus.
- BiologyMolecular marine biology and biotechnology
- 1996
These studies show that DNA vaccination will protect rainbow trout against the lethal effects of IHNV infection.




