Transduction of Cu,Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into mammalian cells.

@article{Kwon2000TransductionOC,
  title={Transduction of Cu,Zn-superoxide dismutase mediated by an HIV-1 Tat protein basic domain into mammalian cells.},
  author={Hyeok Yil Kwon and Won Sik Eum and Hye Won Jang and Jung Hun Kang and Jiyoon Ryu and B Ryong Lee and Li Hua Jin and Jae-Bong Park and Soo Young Choi},
  journal={FEBS letters},
  year={2000},
  volume={485 2-3},
  pages={163-7}
}
A human Cu,Zn-superoxide dismutase (Cu,Zn-SOD) gene was fused with a gene fragment encoding the nine amino acid transactivator of transcription (Tat) protein transduction domain (RKKRRQRRR) of HIV-1 in a bacterial expression vector to produce a genetic in-frame Tat-SOD fusion protein. The expressed and purified Tat-SOD fusion protein in Escherichia coli can enter HeLa cells in a time- and dose-dependent manner when added exogenously in a culture media. Denatured Tat-SOD protein was transduced… CONTINUE READING

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