Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis

@article{Wadia2004TransducibleTF,
  title={Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis},
  author={Jehangir S. Wadia and Radu V Stan and Steven F Dowdy},
  journal={Nature Medicine},
  year={2004},
  volume={10},
  pages={310-315}
}
The TAT protein transduction domain (PTD) has been used to deliver a wide variety of biologically active cargo for the treatment of multiple preclinical disease models, including cancer and stroke. However, the mechanism of transduction remains unknown. Because of the TAT PTD's strong cell-surface binding, early assumptions regarding cellular uptake suggested a direct penetration mechanism across the lipid bilayer by a temperature- and energy-independent process. Here we show, using a… 
Cationic TAT peptide transduction domain enters cells by macropinocytosis.
  • I. Kaplan, J. Wadia, S. Dowdy
  • Biology, Chemistry
    Journal of controlled release : official journal of the Controlled Release Society
  • 2005
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