Transcriptome-wide identification of RNA binding sites by CLIP-seq.

  title={Transcriptome-wide identification of RNA binding sites by CLIP-seq.},
  author={Valentine Murigneux and J{\'e}r{\^o}me Sauli{\`e}re and Hugues Roest Crollius and Herv{\'e} Le Hir},
  volume={63 1},
An emergent strategy for the transcriptome-wide study of protein-RNA interactions is CLIP-seq (crosslinking and immunoprecipitation followed by high-throughput sequencing). We combined CLIP-seq and mRNA-seq to identify direct RNA binding sites of eIF4AIII in human cells. This RNA helicase is a core constituant of the Exon Junction Complex (EJC), a multifunctional protein complex associated with spliced mRNAs in metazoans. Here, we describe the successive steps of the CLIP protocol and the… CONTINUE READING
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