Transcriptome survey reveals increased complexity of the alternative splicing landscape in Arabidopsis.

@article{Marquez2012TranscriptomeSR,
  title={Transcriptome survey reveals increased complexity of the alternative splicing landscape in Arabidopsis.},
  author={Yamile Marquez and John W. S. Brown and Craig Simpson and Andrea Barta and Maria Kalyna},
  journal={Genome research},
  year={2012},
  volume={22 6},
  pages={1184-95}
}
Alternative splicing (AS) is a key regulatory mechanism that contributes to transcriptome and proteome diversity. As very few genome-wide studies analyzing AS in plants are available, we have performed high-throughput sequencing of a normalized cDNA library which resulted in a high coverage transcriptome map of Arabidopsis. We detect ∼150,000 splice junctions derived mostly from typical plant introns, including an eightfold increase in the number of U12 introns (2069). Around 61% of multiexonic… CONTINUE READING
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