Transcriptome sequencing to produce SNP-based genetic maps of onion

  title={Transcriptome sequencing to produce SNP-based genetic maps of onion},
  author={Janejira Duangjit and Borut Bohanec and Agnes P. Chan and Christopher D. Town and Michael J Havey},
  journal={Theoretical and Applied Genetics},
We used the Roche-454 platform to sequence from normalized cDNA libraries from each of two inbred lines of onion (OH1 and 5225). From approximately 1.6 million reads from each inbred, 27,065 and 33,254 cDNA contigs were assembled from OH1 and 5225, respectively. In total, 3,364 well supported single nucleotide polymorphisms (SNPs) on 1,716 cDNA contigs were identified between these two inbreds. One SNP on each of 1,256 contigs was randomly selected for genotyping. OH1 and 5225 were crossed and… CONTINUE READING
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