Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation

@article{Brandenberger2004TranscriptomeCE,
  title={Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation},
  author={Ralph Brandenberger and Henry Wei and Sally Zhang and Shi Lei and Jaji Murage and Gregory J. Fisk and Yan Li and Chunhui Xu and Rixun Fang and Karl J. Guegler and Mahendra S. Rao and Ramumkar Mandalam and Jane S. Lebkowski and Lawrence Walter Stanton},
  journal={Nature Biotechnology},
  year={2004},
  volume={22},
  pages={707-716}
}
Human embryonic stem (hES) cells hold promise for generating an unlimited supply of cells for replacement therapies. To characterize hES cells at the molecular level, we obtained 148,453 expressed sequence tags (ESTs) from undifferentiated hES cells and three differentiated derivative subpopulations. Over 32,000 different transcripts expressed in hES cells were identified, of which more than 16,000 do not match closely any gene in the UniGene public database. Queries to this EST database… 
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