Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation

  title={Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation},
  author={R. Brandenberger and Henry Wei and S. Zhang and Shirley Lei and J. Murage and Gregory J Fisk and Y. Li and C. Xu and R. Fang and K. Guegler and Mahendra S. Rao and R. Mandalam and J. Lebkowski and Lawrence W. Stanton},
  journal={Nature Biotechnology},
  • R. Brandenberger, Henry Wei, +11 authors Lawrence W. Stanton
  • Published 2004
  • Biology, Medicine
  • Nature Biotechnology
  • Human embryonic stem (hES) cells hold promise for generating an unlimited supply of cells for replacement therapies. To characterize hES cells at the molecular level, we obtained 148,453 expressed sequence tags (ESTs) from undifferentiated hES cells and three differentiated derivative subpopulations. Over 32,000 different transcripts expressed in hES cells were identified, of which more than 16,000 do not match closely any gene in the UniGene public database. Queries to this EST database… CONTINUE READING
    327 Citations

    Figures and Topics from this paper.

    Embryonic stem cell proteomics
    • 41
    • PDF
    Comparison of the gene expression profile of undifferentiated human embryonic stem cell lines and differentiating embryoid bodies
    • 114
    • Highly Influenced
    Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene.
    • 58
    Conserved and divergent paths that regulate self-renewal in mouse and human embryonic stem cells.
    • M. Rao
    • Biology, Medicine
    • Developmental biology
    • 2004
    • 170
    • Highly Influenced
    • PDF


    Properties of four human embryonic stem cell lines maintained in a feeder‐free culture system
    • M. Carpenter, E. Rosler, +5 authors M. Rao
    • Biology, Medicine
    • Developmental dynamics : an official publication of the American Association of Anatomists
    • 2004
    • 288
    Synthetic small molecules that control stem cell fate
    • 260
    • PDF
    Role of suppressors of cytokine signaling (Socs) in leukemia inhibitory factor (LIF) ‐dependent embryonic stem cell survival
    • 123
    A role for Wnt signalling in self-renewal of haematopoietic stem cells
    • 2,124
    • PDF
    Wnt proteins are lipid-modified and can act as stem cell growth factors
    • 2,116
    • PDF