Transcriptome analysis provides insights into the molecular mechanisms responsible for evisceration behavior in the sea cucumber Apostichopus japonicus.

  title={Transcriptome analysis provides insights into the molecular mechanisms responsible for evisceration behavior in the sea cucumber Apostichopus japonicus.},
  author={Kui Ding and Libin Zhang and Lina Sun and Chenggang Lin and Qiming Feng and Shuangyan Zhang and Hongsheng Yang and Richard Brinkman and Gang Lin and Zhen Huang},
  journal={Comparative biochemistry and physiology. Part D, Genomics \& proteomics},
  • Kui Ding, Libin Zhang, +7 authors Z. Huang
  • Published 2019
  • Medicine, Biology
  • Comparative biochemistry and physiology. Part D, Genomics & proteomics
The sea cucumber Apostichopus japonicus (Selenka) is a valuable economic species in Southeast Asia. It has many fascinating behavioral characteristics, such as autolysis, aestivation, regeneration, and evisceration, thus it is a notable species for studies of special behaviors. Evisceration and autotomy are controlled by the neural network and involve a complicated physiological process. The occurrence of evisceration behavior in sea cucumbers is strongly related to their environment, and it… Expand
7 Citations
Metabolomic analysis of coelomic fluids reveals the physiological mechanisms underlying evisceration behavior in the sea cucumber Apostichopus japonicus
This study uses ultraperformance liquid chromatography combined with quadrupole time-of-flight mass spectrometry to detect changes in metabolites and metabolic pathways in the process of evisceration behavior of A. japonicus, which could have important implications for the aquaculture and transportation of sea cucumbers. Expand
Comparative Transcriptomes of the Body Wall of Wild and Farmed Sea Cucumber Isostichopus badionotus
The transcriptome of the body wall of wild and farmed I. badionotus has now been studied for the first time by an RNA-Seq approach and gives useful pointers to the underlying problems associated with their aquaculture. Expand
Physiological traits of income breeding strategy in the sea cucumber Apostichopus japonicus
Assessment of physiological traits that underlie income breeding strategy of the A. japonicus sea cucumber showed that income breeding allows all adults, even individuals with poor body condition and small maternal size, to reproduce annually by flexibly adjusting their reproductive output, and dynamic changes of activities of Na+/K+-ATPase, hexokinase and pyruvate kinase suggested that incomebreeding places a metabolic burden on the breeding adults. Expand
Antioxidant Response and Oxidative Stress in the Respiratory Tree of Sea Cucumber (Apostichopus japonicus) Following Exposure to Crude Oil and Chemical Dispersant
Sea cucumber (Apostichopus japonicus) is mainly cultured in the coastal zone, where it is easily threatened by accidental oil spills. Chemical dispersant is one of the efficient oil spill responsesExpand
Combined Effects of Elevated Temperature and Crude Oil Pollution on Oxidative Stress and Apoptosis in Sea Cucumber (Apostichopus japonicus, Selenka)
The results revealed that co-exposure to elevated temperature and crude oil could simulate higher ROS levels and subsequently cause much severer oxidative damage and cellular apoptosis than crude oil alone on sea cucumber. Expand
Molecular cloning, expression and functional analysis of NF-kB1 p105 from sea cucumber Holothuria leucospilota.
Over-expression of HLp105 in HEK293T cells, the apoptosis was inhibited, and the cytokines IL-1β and TNF-α were activated, important for better understanding the function of NF-κB1 p105 in sea cucumber and reveal its involvement in immunoreaction. Expand
Expression of Piwi, MMP, TIMP, and Sox during Gut Regeneration in Holothurian Eupentacta fraudatrix (Holothuroidea, Dendrochirotida)
The expression of several genes involved in gut regeneration in E. fraudatrix were investigated and it was shown that Piwi-positive cells were not involved in the formation of the luminal epithelium of the digestive tube. Expand


Comparative analysis of genes expressed in regenerating intestine and non-eviscerated intestine of Apostichopus japonicus Selenka (Aspidochirotida: Stichopodidae) and cloning of ependymin gene
The regeneration of the intestine of sea cucumber was studied by describing historically the changes that occurred during intestine regeneration on the fifth day after chemically-induced evisceration and revealed that the expression of the genes which might be involved in regeneration was enhanced to some extent after eviscership. Expand
High-Throughput Sequencing Reveals Differential Expression of miRNAs in Intestine from Sea Cucumber during Aestivation
The regulatory role of miRNA in gene expression is an emerging hot new topic in the control of hypometabolism. Sea cucumber aestivation is a complicated physiological process that includes obviousExpand
Large scale gene expression profiling during intestine and body wall regeneration in the sea cucumber Apostichopus japonicus.
Fourteen new candidate gene sequences, discovered for the first time in this study, will greatly enable future research - using the sea cucumber model - into the molecular mechanisms associated with intestine and body wall regeneration. Expand
Metabolic responses to intestine regeneration in sea cucumbers Apostichopus japonicus.
Three important genes were differentially expressed to regulate the levels of betaine and N,N-dimethylglycine during intestine regeneration in sea cucumbers, providing an important basis for studying regenerative mechanisms and developing regenerative matrixes. Expand
Identification of antioxidative oligopeptides derived from autolysis hydrolysates of sea cucumber (Stichopus japonicus) guts
Results suggest that antioxidant oligopeptides derived from the guts of sea cucumber by autolysis method could be utilized for functional foods. Expand
Differences in MITF gene expression and histology between albino and normal sea cucumbers (Apostichopus japonicus Selenka)
Albino A. japonicus have fewer melanocytes and a reduced ability to synthesize melanin, likely because of lower expression of microphthalmia-associated transcription factor. Expand
Transcriptome profiling helps to identify potential and true molecular switches of stealth to brute force behavior in Pectobacterium atrosepticum during systemic colonization of tobacco plants
Genes from the cfa locus responsible for the production of coronafacic acid displayed the strongest induction in the asymptomatic zone relative to the symptomatic one and were shown experimentally to act as the true strategy “switchers” of Pba behavior in planta. Expand
Histological studies on evisceration and regeneration in Aponstichopus japonicus
The result indicated that the A.japonicus started to regenerate immediately after the evisceration, and the respiratory tree trunk and the branched structure were observed on the 2nd day. Expand
Asexual reproduction, evisceration, and regeneration in holothurians (Holothuroidea) from Nha Trang Bay of the South China Sea
The modes of evisceration and regeneration of the gut have been elucidated for ten species of aspidochirotids and it has been shown that the intestine of Holothuria scabra forms from two anlagen rather than from a single one, as previously believed. Expand
RNA-seq Transcriptome Analysis of Panax japonicus, and Its Comparison with Other Panax Species to Identify Potential Genes Involved in the Saponins Biosynthesis
Unigenes identified in this study represent strong candidates for being involved in the triterpenoid saponins biosynthesis, and can serve as a basis for future validation studies. Expand