Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway.

  title={Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway.},
  author={Zhenyan Yang and Lifang Yang and Changkun Liu and Xu-Jie Qin and Haiyang Liu and Jia-Hui Chen and Yunheng Ji},
Steroidal saponins, one of the most diverse groups of plant-derived natural products, elicit biological and pharmacological activities; however, the genes involved in their biosynthesis and the corresponding biosynthetic pathway in monocotyledon plants remain unclear. This study aimed to identify genes involved in the biosynthesis of steroidal saponins by performing a comparative analysis among transcriptomes of Paris polyphylla var. chinensis (PPC), Ypsilandra thibetica (YT), and Polygonatum… 
Transcriptome analysis of Paris polyphylla var. yunnanensis illuminates the biosynthesis and accumulation of steroidal saponins in rhizomes and leaves.
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Effective prediction of biosynthetic pathway genes involved in bioactive polyphyllins in Paris polyphylla.
  • Xin Hua, Wei Song, +6 authors Zheyong Xue
  • Medicine
    Communications biology
  • 2022
The genes in polyphyllins pathway mixed with other steroid biosynthetic genes form an extremely complex biosynthetic network in Paris polyphylla with a giant genome. The lack of genomic data and


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Transcriptomic Comparison Reveals Candidate Genes for Triterpenoid Biosynthesis in Two Closely Related Ilex Species
This study is the first exploration to profile the transcriptome of I. pubescens and identifies a series of candidate genes involved in the biosynthetic pathway of triterpenoid saponins, providing new insight into their biosynthesis at transcriptome level.
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Information generated in this study provides a rich resource for functional analysis of withanolide-specific genes to elucidate chemotype- as well as tissue-specific withanolid biosynthesis, which will help in development of new tools for functional genomics and breeding in Withania.
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High-throughput RNA-Seq analyses of the root, bulb, and leaf of AA, MALs, and FF revealed a strong up-regulation of the saponin downstream pathway, including cytochrome P450, glycosyltransferase, and beta-glucosidase in chromosome 2A.
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Comprehensive genomic dataset created in the current study will serve as a resource for identification of potential candidates for genetic manipulation of targeted bioactive metabolites and also contribute for development of functionally relevant molecular marker resource to expedite molecular breeding and conservation efforts in T. govanianum.
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