Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway.

@article{Yang2019TranscriptomeAO,
  title={Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway.},
  author={Zhenyan Yang and Lifang Yang and Changkun Liu and Xu-Jie Qin and Haiyang Liu and Jia-Hui Chen and Yunheng Ji},
  journal={Fitoterapia},
  year={2019},
  volume={135},
  pages={
          52-63
        }
}
Steroidal saponins, one of the most diverse groups of plant-derived natural products, elicit biological and pharmacological activities; however, the genes involved in their biosynthesis and the corresponding biosynthetic pathway in monocotyledon plants remain unclear. This study aimed to identify genes involved in the biosynthesis of steroidal saponins by performing a comparative analysis among transcriptomes of Paris polyphylla var. chinensis (PPC), Ypsilandra thibetica (YT), and Polygonatum… 
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Effective prediction of biosynthetic pathway genes involved in bioactive polyphyllins in Paris polyphylla.
  • Xin Hua, Wei Song, +6 authors Zheyong Xue
  • Medicine
    Communications biology
  • 2022
The genes in polyphyllins pathway mixed with other steroid biosynthetic genes form an extremely complex biosynthetic network in Paris polyphylla with a giant genome. The lack of genomic data and

References

SHOWING 1-10 OF 75 REFERENCES
De novo transcriptome sequencing of Momordica cochinchinensis to identify genes involved in the carotenoid biosynthesis
TLDR
This study has revealed putative phytoene synthase, 15-cis-phytone desaturase, zeta-carotene desaturases, carotenoid isomerase and lycopene epsilon cyclase might be key factors for controlling carotanoid contents during aril ripening.
Transcriptomic Comparison Reveals Candidate Genes for Triterpenoid Biosynthesis in Two Closely Related Ilex Species
TLDR
This study is the first exploration to profile the transcriptome of I. pubescens and identifies a series of candidate genes involved in the biosynthetic pathway of triterpenoid saponins, providing new insight into their biosynthesis at transcriptome level.
Comparative transcriptome analysis of different chemotypes elucidates withanolide biosynthesis pathway from medicinal plant Withania somnifera
TLDR
Information generated in this study provides a rich resource for functional analysis of withanolide-specific genes to elucidate chemotype- as well as tissue-specific withanolid biosynthesis, which will help in development of new tools for functional genomics and breeding in Withania.
RNA-sequencing-based transcriptome and biochemical analyses of steroidal saponin pathway in a complete set of Allium fistulosum—A. cepa monosomic addition lines
TLDR
High-throughput RNA-Seq analyses of the root, bulb, and leaf of AA, MALs, and FF revealed a strong up-regulation of the saponin downstream pathway, including cytochrome P450, glycosyltransferase, and beta-glucosidase in chromosome 2A.
De novo leaf and root transcriptome analysis identified novel genes involved in Steroidal sapogenin biosynthesis in Asparagus racemosus
TLDR
A large set of cDNA unigenes from A. racemosus leaf and root tissue are identified using next generation sequencing technology and number of root specific transcripts showing homology with saponin biosynthetic pathway are identified.
Spatial transcriptome analysis provides insights of key gene(s) involved in steroidal saponin biosynthesis in medicinally important herb Trillium govanianum
TLDR
Comprehensive genomic dataset created in the current study will serve as a resource for identification of potential candidates for genetic manipulation of targeted bioactive metabolites and also contribute for development of functionally relevant molecular marker resource to expedite molecular breeding and conservation efforts in T. govanianum.
Identification, characterization and expression analysis of genes involved in steroidal saponin biosynthesis in Dracaena cambodiana
Dracaena cambodiana is a traditional medicinal plant used for producing dragon’s blood. The plants and dragon’s blood of D. cambodiana contain a rich variety of steroidal saponins. However, little is
An efficient approach to finding Siraitia grosvenorii triterpene biosynthetic genes by RNA-seq and digital gene expression analysis
TLDR
A combination of RNA-seq and DGE analysis based on the next generation sequencing technology was shown to be a powerful method for identifying candidate genes encoding enzymes responsible for the biosynthesis of novel secondary metabolites in a non-model plant.
Divergent evolution of oxidosqualene cyclases in plants.
TLDR
The phylogenetic analysis suggests that expansion of OSC members in higher plants has occurred mainly through tandem duplication followed by positive selection and diversifying evolution, and consolidated the previous suggestion that dicot triterpene synthases have been derived from an ancestral lanosterol synthase instead of directly from their cycloartenol synthases.
Molecular activities, biosynthesis and evolution of triterpenoid saponins.
TLDR
This review summarizes current knowledge of triterpenoid saponin biosynthesis in plants, molecular activities, evolutionary aspects and perspectives for further gene discovery.
...
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2
3
4
5
...