Transcriptome-Wide Mapping of N⁶-Methyladenosine by m⁶A-Seq.

Abstract

A detailed protocol for isolation and sequencing of an enriched population of m(6)A-methylated RNA fragments to create m(6)A methylome maps is outlined. Our approach was developed to fill a void that existed because of a lack of methods for the detection of m(6)A in RNA in an unbiased, high-throughput, and high-resolution manner. This method integrates… (More)
DOI: 10.1016/bs.mie.2015.03.001

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