Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing.

@article{Saadeh2016TranscriptomeWideIO,
  title={Transcriptome-Wide Identification of Hfq-Associated RNAs in Brucella suis by Deep Sequencing.},
  author={Bashir Saadeh and Clayton C. Caswell and Yanjie Chao and Philippe Berta and Alice R. Wattam and Roy Martin Roop and David O'Callaghan},
  journal={Journal of bacteriology},
  year={2016},
  volume={198 3},
  pages={427-35}
}
UNLABELLED Recent breakthroughs in next-generation sequencing technologies have led to the identification of small noncoding RNAs (sRNAs) as a new important class of regulatory molecules. In prokaryotes, sRNAs are often bound to the chaperone protein Hfq, which allows them to interact with their partner mRNA(s). We screened the genome of the zoonotic and human pathogen Brucella suis 1330 for the presence of this class of RNAs. We designed a coimmunoprecipitation strategy that relies on the use… CONTINUE READING