Transcriptional repressor CopR: the structured acidic C terminus is important for protein stability.

@article{Kuhn2000TranscriptionalRC,
  title={Transcriptional repressor CopR: the structured acidic C terminus is important for protein stability.},
  author={Kornelia Kuhn and Katrin Steinmetzer and Sabine Brantl},
  journal={Journal of molecular biology},
  year={2000},
  volume={300 5},
  pages={
          1021-31
        }
}
The transcriptional repressor CopR is one of the two copy-number control components of plasmid pIP501. CopR binds as a dimer at two consecutive major grooves on the same face of the DNA. Previously, equilibrium dissociation constants of CopR dimers and the CopR-DNA complex and the intracellular CopR concentration were calculated. Amino acid residues involved in DNA binding and dimerization were determined. Here, we provide a detailed analysis of the acidic C terminus of CopR. A series of C… CONTINUE READING
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