Transcriptional regulatory elements stimulate recombination in extrachromosomal substrates carrying immunoglobulin switch-region sequences.

@article{Leung1992TranscriptionalRE,
  title={Transcriptional regulatory elements stimulate recombination in extrachromosomal substrates carrying immunoglobulin switch-region sequences.},
  author={Hoi Chung Leung and Nancy Maizels},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 9},
  pages={4154-8}
}
We have developed a sensitive genetic assay to analyze DNA sequences and regulatory elements required for immunoglobulin heavy chain isotype switch recombination. Recombination substrates containing mu and gamma 3 chain switch (S)-region sequences, S mu and S gamma 3, are transiently introduced into primary murine B cells cultured with lipopolysaccharide to induce isotype switching. Recombination involving S-region sequences deletes a conditionally lethal marker, the leftward promoter of phage… CONTINUE READING

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