Transcriptional regulation of muscle-specific genes during myoblast differentiation.

  title={Transcriptional regulation of muscle-specific genes during myoblast differentiation.},
  author={Teruhiko Shimokawa and Miyuki Kato and Osamu Ezaki and Shinichi Hashimoto},
  journal={Biochemical and biophysical research communications},
  volume={246 1},
A rapid, highly sensitive method to determine the mRNA level of muscle-specific markers using TaqMan PCR analysis was developed and used to study sequential gene regulation of myoblasts during induced differentiation of C2C12 cells. mRNA levels of muscle regulatory factor (MRF) myogenin, alpha-actin, thermoregulatory uncoupling protein UCP2 and glucose transporter isotype glut4 increased rapidly during early stage differentiation. In contrast, myf5, beta-actin, UCP1 and glut1 mRNA levels… CONTINUE READING

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