Transcriptional regulation of IGF-I expression in skeletal muscle.

  title={Transcriptional regulation of IGF-I expression in skeletal muscle.},
  author={Gary E McCall and David L. Allen and Farhang Haddad and Kenneth M. Baldwin},
  journal={American journal of physiology. Cell physiology},
  volume={285 4},
The present study investigated the role of transcription in the regulation of insulin-like growth factor (IGF)-I expression in skeletal muscle. RT-PCR was used to determine endogenous expression of IGF-I pre-mRNA and mRNA in control (Con) and functionally overloaded (FO) rat plantaris. The transcriptional activities of five different-length IGF-I promoter fragments controlling transcription of a firefly luciferase (FLuc) reporter gene were tested in vitro by transfection of myoblasts or in vivo… CONTINUE READING