Transcriptional profiling of the Arabidopsis embryo.

  title={Transcriptional profiling of the Arabidopsis embryo.},
  author={Matthew W. B. Spencer and Stuart A. Casson and Keith Lindsey},
  journal={Plant physiology},
  volume={143 2},
We have used laser-capture microdissection to isolate RNA from discrete tissues of globular, heart, and torpedo stage embryos of Arabidopsis (Arabidopsis thaliana). This was amplified and analyzed by DNA microarray using the Affymetrix ATH1 GeneChip, representing approximately 22,800 Arabidopsis genes. Cluster analysis showed that spatial differences in gene expression were less significant than temporal differences. Time course analysis reveals the dynamics and complexity of gene expression in… CONTINUE READING
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