Transcriptional memory inherited from donor cells is a developmental defect of bovine cloned embryos

@article{Zhou2020TranscriptionalMI,
  title={Transcriptional memory inherited from donor cells is a developmental defect of bovine cloned embryos},
  author={Chuan Jie Zhou and Jingcheng Zhang and M. Zhang and De-jing Wang and Yi Ma and Yong Sheng Wang and Yizhi Wang and Yuemeng Huang and Yong Zhang},
  journal={The FASEB Journal},
  year={2020},
  volume={34},
  pages={1637 - 1651}
}
Studies on the effects of transcriptional memory on clone reprogramming in mammals are limited. In the present study, we observed higher levels of active histone H3 lysine 4 trimethylation (H3K4me3 and 5‐hydroxymethylcytosine) and repressive (5‐methylcytosine) epigenetic modifications in bovine early cloned embryos than in in vitro fertilized embryos. We hypothesized that aberrant epigenetic modification may result in transcriptional disorders in bovine somatic cell nuclear transfer (SCNT… 

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Although large-scale aberrations in expression of mRNAs were evident during the maternal-to-embryonic transition in cattle scNT embryos, these changes were not consistently correlated with changes in miRNA expression at the same developmental stage, suggesting that other mechanisms controlling gene expression may be involved.

Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b

Advance in the Role of Epigenetic Reprogramming in Somatic Cell Nuclear Transfer-Mediated Embryonic Development

The repprogramming process and reprogramming defects of several important epigenetic marks are reviewed and epigenetic barriers that may lead to the aberrant reprograming are highlighted and insights into improving the efficiency and quality of SCNT-mediated reprogrammers are given.

Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How

This review describes in detail where, when, and how manipulations of the epigenome of donor cells and reconstructed SCNT embryos can be performed to optimize the process of molecular reprogramming and the outcome of nuclear transfer cloning.

25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER Epigenetic abnormalities associated with somatic cell nuclear transfer

It is known that the imprinting memories in the donor genome, whether canonical (DNA-methylation-dependent) or noncanonical (H3K27me3-dependent), are not reprogrammed by SCNT, and SCNT-derived embryos have the normal canonical imprinting and the erased noncanonicals imprinting, both being inherited from the donor cells.

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