Transcriptional memory inherited from donor cells is a developmental defect of bovine cloned embryos

  title={Transcriptional memory inherited from donor cells is a developmental defect of bovine cloned embryos},
  author={Chuan Jie Zhou and Jingcheng Zhang and M. Zhang and De-jing Wang and Yi Ma and Yong Sheng Wang and Yizhi Wang and Yuemeng Huang and Yong Zhang},
  journal={The FASEB Journal},
  pages={1637 - 1651}
Studies on the effects of transcriptional memory on clone reprogramming in mammals are limited. In the present study, we observed higher levels of active histone H3 lysine 4 trimethylation (H3K4me3 and 5‐hydroxymethylcytosine) and repressive (5‐methylcytosine) epigenetic modifications in bovine early cloned embryos than in in vitro fertilized embryos. We hypothesized that aberrant epigenetic modification may result in transcriptional disorders in bovine somatic cell nuclear transfer (SCNT… 

Characterization of transcriptional activity during ZGA in mammalian SCNT embryo

characterization of transcriptional activity and identify critical genes in mammalian SCNT embryo and identified three modules correlated with the development of SCNT embryos in mice and screened 288 genes likely critical for SCNT reprogramming using weighted gene correlation network analysis.

ZFP57 regulates DNA methylation of imprinted genes to facilitate embryonic development of somatic cell nuclear transfer embryos in Holstein cows.

It is concluded that overexpression of ZFP57 in donor cells provided an effective method for enhancing nuclear reprogramming and developmental potential in SCNT embryos.

Reprogramming barriers in bovine cells nuclear transfer revealed by single‐cell RNA‐seq analysis

Results suggested partial reprogrammed 3F biPS cells as donors for bovine nuclear transfer hindered the reprogramming of nuclear transfer embryos, and revealed the abnormal gene expression and pathway activation of SCNT embryos.

Comparing mRNA and sncRNA profiles during the maternal-to-embryonic transition in bovine IVF and scNT embryos

Although large-scale aberrations in expression of mRNAs were evident during the maternal-to-embryonic transition in cattle scNT embryos, these changes were not consistently correlated with changes in miRNA expression at the same developmental stage, suggesting that other mechanisms controlling gene expression may be involved.

Long non-coding RNA lnc_3712 impedes nuclear reprogramming via repressing Kdm5b

Advance in the Role of Epigenetic Reprogramming in Somatic Cell Nuclear Transfer-Mediated Embryonic Development

The repprogramming process and reprogramming defects of several important epigenetic marks are reviewed and epigenetic barriers that may lead to the aberrant reprograming are highlighted and insights into improving the efficiency and quality of SCNT-mediated reprogrammers are given.

Manipulating the Epigenome in Nuclear Transfer Cloning: Where, When and How

This review describes in detail where, when, and how manipulations of the epigenome of donor cells and reconstructed SCNT embryos can be performed to optimize the process of molecular reprogramming and the outcome of nuclear transfer cloning.

25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER Epigenetic abnormalities associated with somatic cell nuclear transfer

It is known that the imprinting memories in the donor genome, whether canonical (DNA-methylation-dependent) or noncanonical (H3K27me3-dependent), are not reprogrammed by SCNT, and SCNT-derived embryos have the normal canonical imprinting and the erased noncanonicals imprinting, both being inherited from the donor cells.

Linker histone H1FOO is required for bovine preimplantation development by regulating lineage specification and nucleosome assembly

It is proposed that H1FOO controls the proper chromatin structure that is crucial for the fidelity of cell polarization and lineage specification during bovine early development.



Aberrant methylation of donor genome in cloned bovine embryos

Evidence is provided that cloned bovine embryos may have impaired epigenetic reprogramming capabilities and highly aberrant methylation patterns in various genomic regions of cloned embryos, indicating that the developmental anomalies ofCloned embryos could be due to incomplete epigeneticReprogramming of donor genomic DNA.

Transcriptional defects and reprogramming barriers in somatic cell nuclear reprogramming as revealed by single-embryo RNA sequencing

These embryonic transcriptome blueprints provide new data for further mechanistic studies of somatic nuclear reprogramming and may improve the efficiency of somatics cell nuclear transfer.

H3K9 demethylase KDM4E is an epigenetic regulator for bovine embryonic development and a defective factor for nuclear reprogramming

RNA-dependent KDM4E chromatin localization is essential for active H3K9 demethylation during bovine embryonic genome activation, an observation that could improve outcomes in cloned cattle production.

H3K27me3 is an epigenetic barrier while KDM6A overexpression improves nuclear reprogramming efficiency

  • C. ZhouYizhi Wang Yong Zhang
  • Biology
    FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 2019
It is shown that SCNT embryos exhibit global H3K27me3 hypermethylation from the 2‐ to 8‐cell stage and that its removal by ectopically expressed KDM6A lysine demethylase (KDM)6A greatly improves nuclear reprogramming efficiency.

Incomplete DNA methylation underlies a transcriptional memory of somatic cells in human iPS cells

Human induced pluripotent stem (iPS) cells are remarkably similar to embryonic stem (ES) cells, but recent reports indicate that there may be important differences between them. We carried out a

Epigenetic memory of active gene transcription is inherited through somatic cell nuclear transfer.

  • R. K. NgJ. Gurdon
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2005
It is concluded that an epigenetic memory is established in differentiating somatic cells and applies to genes that are in a transcriptionally active state in some nuclear transplant embryos.

Histone H3 lysine 27 trimethylation acts as an epigenetic barrier in porcine nuclear reprogramming.

H3K 27me3 acts as an epigenetic barrier in SCNT and iPS reprogramming, and reduction of H3K27me3 level in donor cells and in early reprograming phase can enhance both porcine SCNT or iPS efficiency.