Transcriptional control of the succinate dehydrogenase operon sdhCAB of Corynebacterium glutamicum by the cAMP-dependent regulator GlxR and the LuxR-type regulator RamA.

@article{Bussmann2009TranscriptionalCO,
  title={Transcriptional control of the succinate dehydrogenase operon sdhCAB of Corynebacterium glutamicum by the cAMP-dependent regulator GlxR and the LuxR-type regulator RamA.},
  author={Michael Bussmann and Denise Emer and Sonja Hasenbein and Sabine Degraf and Bernhard J Eikmanns and Michael Bott},
  journal={Journal of biotechnology},
  year={2009},
  volume={143 3},
  pages={173-82}
}
In experiments performed to identify transcriptional regulators of the tricarboxylic acid cycle of Corynebacterium glutamicum, the cAMP-dependent regulator GlxR and the regulators of acetate metabolism RamA and RamB were enriched by DNA affinity chromatography with the promoter region of the sdhCAB operon encoding succinate dehydrogenase. The binding of purified GlxR, RamA and RamB was verified by electrophoretic mobility shift assays and the regulatory effects of these proteins on sdhCAB gene… CONTINUE READING

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