Transcriptional control of the Pseudomonas putida TOL plasmid catabolic pathways

@article{Marqus1993TranscriptionalCO,
  title={Transcriptional control of the Pseudomonas putida TOL plasmid catabolic pathways},
  author={Silvia Marqu{\'e}s and Juan L. Ramos},
  journal={Molecular Microbiology},
  year={1993},
  volume={9}
}
  • S. Marqués, Juan L. Ramos
  • Published 1993
  • Biology, Medicine
  • Molecular Microbiology
TOL plasmid pWWO of Pseudomonas putida contains two operons that specify a pathway for the degradation of aromatic hydrocarbons. The upper pathway operon encodes the enzymes for the oxidation of toluene/xylenes to benzoate/toluates, and the meta‐cleavage pathway operon encodes the enzymes for the further oxidation of these compounds to Krebs cycle intermediates. Their expression is controlled by the gene products of two divergently transcribed regulatory genes, xylR and xylS. The XylR protein… Expand
Transcriptional control of the multiple catabolic pathways encoded on the TOL plasmid pWW53 of Pseudomonas putida MT53
TLDR
The TOL plasmid pWW53 encodes a catabolic pathway for the metabolism of toluene and bears an upper-pathway operon for the oxidation of toLUene to benzoate and a copy of the gene that encodes regulatory protein XylR, which stimulation of transcription was found to be mediated by XylS1. Expand
Transcriptional control of the Pseudomonas TOL plasmid catabolic operons is achieved through an interplay of host factors and plasmid-encoded regulators.
The xyl genes of Pseudomonas putida TOL plasmid that specify catabolism of toluene and xylenes are organized in four transcriptional units: the upper-operon xylUWCAMBN for conversion ofExpand
Cross-regulation by XylR and DmpR activators of Pseudomonas putida suggests that transcriptional control of biodegradative operons evolves independently of catabolic genes
TLDR
Genetic evidence is presented that the two activators can functionally substitute each other in the regulation of their corresponding promoters by binding the same upstream DNA segment. Expand
Transcriptional induction kinetics from the promoters of the catabolic pathways of TOL plasmid pWW0 of Pseudomonas putida for metabolism of aromatics
TLDR
The kinetics of mRNA synthesis from the four Pseudomonas putida pWW0 plasmid promoters involved in degradation of xylenes and methylbenzyl alcohols via toluates are determined. Expand
Involvement of IHF protein in expression of the Ps promoter of the Pseudomonas putida TOL plasmid
TLDR
DNase I footprint experiments showed that one of these sites, which overlaps the recognition site for XylR activator, as well as an AT-rich region comprising the Ps promoter consensus were protected by integration host factor (IHF). Expand
Genetic evidence of separate repressor and activator activities of the XylR regulator of the TOL plasmid, pWWO, of Pseudomonas putida
TLDR
It is shown that formation of a nucleoprotein complex involving the polymerase bound to the divergent promoter Ps is not required for downregulation of Pr, and sensu stricto XylR is defined as a transcriptional repressor, independently of its activator role in other promoters. Expand
Expression of the TOL plasmid xylS gene in Pseudomonas putida occurs from a alpha 70-dependent promoter or from alpha 70- and alpha 54-dependent tandem promoters according to the compound used for growth
TLDR
It is shown here that in bacteria growing on glycerol or alkylbenzoates, the xylS gene is expressed at a low but constitutive level from a newly found sigma 70-dependent promoter called Ps2, which explains why sigma 54-deficient P. putida strain bearing a TOL plasmid with a knocked-out xylR gene, can grow on alkyLbenzoate. Expand
Cross talk between catabolic pathways in Pseudomonas putida: XylS-dependent and -independent activation of the TOL meta operon requires the same cis-acting sequences within the Pm promoter
TLDR
A series of deletions and mutations at the Pm promoter sequence showed the same overall pattern of responsiveness to benzoate with and without XylS, thus providing genetic evidence that the same promoter structure is recognized and activated by at least two different regulators. Expand
The sigma 54-dependent promoter Ps of the TOL plasmid of Pseudomonas putida requires HU for transcriptional activation in vivo by XylR
TLDR
In vivo analysis of transcriptional activity in various genetic backgrounds suggests that the looping out of intervening DNA sequences in Ps would result from the exacerbation of a preexisting static bend within the region, assisted by the histone-like protein HU. Expand
Activation of the transcriptional regulator XylR of Pseudomonas putida by release of repression between functional domains
TLDR
Data indicate that a key event in the activation of XyIR by toluene/xylenes is the release of the repression caused by the Adomain of the protein on surfaces located at the central domain of the regulator. Expand
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References

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Regulatory circuits controlling transcription of TOL plasmid operon encoding meta‐cleavage pathway for degradation of alkylbenzoates by Pseudomonas
TOL plasmid PWWO of Pseudomonas putida contains two operons that specify a pathway for the degradation of aromatic hydrocarbons. The ‘upper’ operon encodes enzymes for the oxidation of toluene toExpand
Upstream regulatory sequence for transcriptional activator XylR in the first operon of xylene metabolism on the TOL plasmid.
TLDR
DNA-loop formation through protein-protein interaction between XylR protein attached to the upstream sequence and the NtrA-containing RNA polymerase bound by the promoter sequence was suggested for activation of the operon transcription. Expand
Transcription of the TOL plasmid toluate catabolic pathway operon of Pseudomonas putida is determined by a pair of co‐ordinately and positively regulated overlapping promoters.
TLDR
Comparison of the promoter sequences obtained suggests a tentative consensus sequence for promoters of P. putida which is significantly different from that of E. coli. Expand
An upstream XylR‐ and IHF‐induced nucleoprotein complex regulates the sigma 54‐dependent Pu promoter of TOL plasmid.
TLDR
In vivo monitoring of the activity of a Pu‐lacZ fusion in E. coli strains with different genetic backgrounds demonstrated that integration host factor (IHF) is involved in Pu regulation and that hyperproduction of the XylR protein leads to a decrease of Pu activity in a manner in which deletion of the putative DNA‐binding domain of theXylR does not impair its inhibitory effect when hyperproduced. Expand
Characterization of five genes in the upper-pathway operon of TOL plasmid pWW0 from Pseudomonas putida and identification of the gene products
TLDR
The upper operon of the TOL plasmid pWW0 of Pseudomonas putida encodes a set of enzymes which transform toluene and xylenes to benzoate and toluates and synthesizes a 52-kilodalton polypeptide of unknown function. Expand
Nucleotide sequence surrounding transcription initiation site of xylABC operon on TOL plasmid of Pseudomonas putida.
The xylABC operon on the TOL plasmid directs the synthesis of enzymes for conversion of toluene to benzoate and is positively controlled by the regulatory gene xylR. In the study here the nucleotideExpand
Molecular and functional analysis of the TOL plasmid pWWO from Pseudomonas putida and cloning of genes for the entire regulated aromatic ring meta cleavage pathway.
TLDR
Evidence is presented that suggests the promoter operator of the meta pathway gene functions less effectively with the RNA polymerase or xylS product of E. coli than with the enzyme or product of P. putida. Expand
Molecular cloning of gene xylS of the TOL plasmid: evidence for positive regulation of the xylDEGF operon by xylS
TLDR
The xylDEGF operon and the regulatory gene xylS of the TOL plasmid found in Pseudomonas putida mt-2 were cloned onto Escherichia coli vector plasmids, and the map positions of xylG and xylF were determined. Expand
Activation of the Pseudomonas TOL plasmid upper pathway operon. Identification of binding sites for the positive regulator XylR and for integration host factor protein.
TLDR
XylR-dependent stimulation of transcription from the Pseudomonas TOL upper pathway promoter was examined using deletions, insertions, and in vivo dimethyl sulfate footprinting, which revealed changes in the methylation pattern of G and T occurred in the -50 to -90 region, which is probably occupied by integration host factor (IHF) protein. Expand
Gene order of the TOL catabolic plasmid upper pathway operon and oxidation of both toluene and benzyl alcohol by the xylA product
TLDR
Determination of upper pathway enzyme levels in bacteria carrying Tn5 insertion mutant derivatives of plasmid pWW0-161 has shown that the genes for upper pathway enzymes are organized in an operon with the following order: promoter-xylC (benzaldehyde dehydrogenase gene[s],xylA (xylene oxygenase gene)[s], andxylB (benZyl alcohol dehydrogen enzyme gene). Expand
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