Transcriptional coactivator EDF-1 is required for PPARγ-stimulated adipogenesis

  title={Transcriptional coactivator EDF-1 is required for PPARγ-stimulated adipogenesis},
  author={Marzia Leidi and Massimo Mariotti and Jeanette A. M. Maier},
  journal={Cellular and Molecular Life Sciences},
Peroxisome proliferator-activated receptor-γ (PPARγ) is essential for adipogenesis. Since EDF-1 is a cofactor of PPARγ, we investigated the molecular cross-talk between EDF-1 and PPARγ in adipogenesis. While EDF-1 was not modulated during differentiation of 3T3-L1 cells, it co-immunoprecipitated with PPARγ. Silencing EDF-1 by shRNAs inhibited the differentiation in adipocytes of 3T3-L1 cells, as detected by the staining of intracellular triglycerides and the expression of the PPARγ target gene… CONTINUE READING