Transcriptional and post-transcriptional control of polynucleotide phosphorylase during cold acclimation in Escherichia coli.

@article{Zangrossi2000TranscriptionalAP,
  title={Transcriptional and post-transcriptional control of polynucleotide phosphorylase during cold acclimation in Escherichia coli.},
  author={Sandro Zangrossi and Federica Briani and Daniela Ghisotti and Maria Elena Regonesi and Paolo Tortora and Gianni Deh{\`o}},
  journal={Molecular microbiology},
  year={2000},
  volume={36 6},
  pages={1470-80}
}
Polynucleotide phosphorylase (PNPase, polyribonucleotide nucleotidyltransferase, EC 2.7.7.8) is one of the cold shock-induced proteins in Escherichia coli and pnp, the gene encoding it, is essential for growth at low temperatures. We have analysed the expression of pnp upon cold shock and found a dramatic transient variation of pnp transcription profile: within the first hour after temperature downshift the amount of pnp transcripts detectable by Northern blotting increased more than 10-fold… CONTINUE READING

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