Transcriptional analysis of the recA gene in Streptomyces rimosus: identification of the new type of promoter.

@article{Ahel2002TranscriptionalAO,
  title={Transcriptional analysis of the recA gene in Streptomyces rimosus: identification of the new type of promoter.},
  author={Ivan Ahel and Dusica Vujaklija and Andreja Miko{\vc} and V. Gamulin},
  journal={FEMS microbiology letters},
  year={2002},
  volume={209 1},
  pages={
          133-7
        }
}
Using primer-extension analysis we identified two transcription start sites for the recA gene in Streptomyces rimosus. A longer, weak transcript is initiated from the distal SEP promoter that contains a Cheo box like sequence: GAAC-N4-ATTC. However, the major start site of transcription is a G at position -36 and this shorter transcript significantly increases in response to DNA damage by UV-light. The -35 box (TTGTCA) and -10 box (TAGCGT) of the strong recA promoter are only 11 bp apart and… CONTINUE READING

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