Transcriptional analysis of purified histone acetyltransferase complexes.

@article{Steger1999TranscriptionalAO,
  title={Transcriptional analysis of purified histone acetyltransferase complexes.},
  author={David J Steger and Jerry L. Workman},
  journal={Methods},
  year={1999},
  volume={19 3},
  pages={410-6}
}
Acetylation of lysine residues within the amino-terminal tails of the core histone proteins is strongly correlated to the regulation of gene transcription in vivo. To directly study the effects of histone acetylation on transcription, we have developed a biochemical system examining the regulation of RNA polymerase II-directed transcription by native histone acetyltransferases (HATs). For the promoter sequences investigated, it has been demonstrated that HATs facilitate transcription from… CONTINUE READING