Transcriptional analysis and proteomics of the holomycin gene cluster in overproducer mutants of Streptomyces clavuligerus.

@article{RoblesReglero2013TranscriptionalAA,
  title={Transcriptional analysis and proteomics of the holomycin gene cluster in overproducer mutants of Streptomyces clavuligerus.},
  author={V. Robles-Reglero and Irene Santamarta and Rub{\'e}n {\'A}lvarez-{\'A}lvarez and Juan F. Martin and Paloma Liras},
  journal={Journal of biotechnology},
  year={2013},
  volume={163 1},
  pages={69-76}
}
Expression of the holomycin biosynthesis genes (hlm) has been studied in the wild type strain Streptomyces clavuligerus ATCC 27064 and holomycin overproducer mutants. RT-PCR transcription analysis of S. clavuligerus oppA2::aph showed a higher transcription of the hlmA, B, C, D, E, F, G, H, I and hlmL genes, a slightly lower expression for hlmK and no significant differences for the transcription of the two putative regulatory genes, hlmM and hlmJ, in relation to the wild type strain… CONTINUE READING

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