Transcriptional activity of paddy soil bacterial communities.

@article{Shrestha2009TranscriptionalAO,
  title={Transcriptional activity of paddy soil bacterial communities.},
  author={Pravin Malla Shrestha and Michael Kube and Richard Reinhardt and Werner Liesack},
  journal={Environmental microbiology},
  year={2009},
  volume={11 4},
  pages={
          960-70
        }
}
Bulk mRNA was used to explore the transcriptional activity of bacterial communities in oxic versus anoxic paddy soil. Two microbial cDNA libraries were constructed from composite samples using semi-randomly primed RT-PCR. cDNAs averaged 500-600 bp in length and were treated as expressed sequence tags (ESTs). Clustering analysis of 805 random cDNAs resulted in 179 and 155 different ESTs for the oxic and anoxic zones respectively. Using an E-value threshold of e(-10), a total of 218 different… CONTINUE READING

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