Transcriptional activation of the pathway‐specific regulator of the actinorhodin biosynthetic genes in Streptomyces coelicolor

@article{Uguru2005TranscriptionalAO,
  title={Transcriptional activation of the pathway‐specific regulator of the actinorhodin biosynthetic genes in Streptomyces coelicolor},
  author={Gabriel C Uguru and Karen Stephens and Jonathan A Stead and Jane E Towle and S. Baumberg and Kenneth J McDowall},
  journal={Molecular Microbiology},
  year={2005},
  volume={58}
}
The Streptomyces produce a plethora of secondary metabolites including antibiotics and undergo a complex developmental cycle. As a means of establishing the pathways that regulate secondary metabolite production by this important bacterial genus, the model species Streptomyces coelicolor and its relatives have been the subject of several genetic screens. However, despite the identification and characterization of numerous genes that affect antibiotic production, there is still no overall… Expand
Regulation of a Novel Gene Cluster Involved in Secondary Metabolite Production in Streptomyces coelicolor
TLDR
Evidence is provided for the contribution of a novel genetic locus to antibiotic production in Streptomyces coelicolor by overexpression of a gene cluster comprising four protein-encoding genes (abeABCD) and an antisense RNA-encoded gene (α-abeA). Expand
The global role of ppGpp synthesis in morphological differentiation and antibiotic production in Streptomyces coelicolor A3(2)
TLDR
In S. coelicolor, ppGpp synthesis influences the expression of several genomic elements that are particularly characteristic of streptomycete biology, notably antibiotic gene clusters, conservons, and morphogenetic proteins. Expand
Characterization of rrdA, a TetR Family Protein Gene Involved in the Regulation of Secondary Metabolism in Streptomyces coelicolor
TLDR
An in vivo transposition system was used to identify novel regulators that influence Red production in Streptomyces coelicolor M145 and showed that RrdA negatively regulated Red production by controlling redD mRNA abundance, while no change was observed at the transcript level of the Act-specific activator gene, actII-orf4. Expand
Molecular Regulation of Antibiotic Biosynthesis in Streptomyces
TLDR
The physiological signals and regulatory mechanisms may be of practical importance for the activation of the many cryptic secondary metabolic gene cluster pathways revealed by recent sequencing of numerous Streptomyces genomes. Expand
Gene networks regulating secondary metabolism in actinomycetes: Pleiotropic regulators
TLDR
The study of regulatory genes for antibiotic biosynthesis will provide insights into the process of evolution of complex regulatory systems that coordinate the expression of gene operons, clusters, and regulons, involved in the control of the secondary metabolism and morphogenesis of actinomycetes. Expand
6S RNA modulates growth and antibiotic production in Streptomyces coelicolor
TLDR
It is concluded that 6S RNA contributes to the optimization of cellular adaptation and is an important factor involved in the regulation of growth and expression of key genes for the biosynthesis of actinorhodin. Expand
Genome-Wide Mutagenesis Links Multiple Metabolic Pathways with Actinorhodin Production in Streptomyces coelicolor
TLDR
Genome-wide mutagenesis can identify novel genes and pathways that impact antibiotic levels, potentially aiding in engineering strains to optimize the production of antibiotics in Streptomyces. Expand
Carbon Catabolite Regulation of Secondary Metabolite Formation and Morphological Differentiation in Streptomyces coelicolor
TLDR
This is the first global transcriptomic approach performed to understand the molecular basis of the glucose effect on the synthesis of secondary metabolism and differentiation in the genus Streptomyces and the results are opening new avenues for further exploration. Expand
Repression of Antibiotic Production and Sporulation in Streptomyces coelicolor by Overexpression of a TetR Family Transcriptional Regulator
TLDR
The direct and functional interaction of SCO3201 with the promoter region of scbA, a gene under the positive control of the TetR-like regulator, ScbR, was indeed demonstrated by in vitro as well as in vivo approaches. Expand
Identification of a Gene Negatively Affecting Antibiotic Production and Morphological Differentiation in Streptomyces coelicolor A3(2)
TLDR
The subcloning of SC7A1 insert DNA resulted in the identification of the open reading frame SCO5582 as nsdA, a gene negatively affecting Streptomyces differentiation that caused the overproduction of spores and of three of four known S. coelicolor antibiotics of quite different chemical types. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 99 REFERENCES
Stationary‐phase production of the antibiotic actinorhodin in Streptomyces coelicolor A3(2) is transcriptionally regulated
TLDR
Production of actinorhodin, a polyketide antibiotic made by Streptomyces coelicolor A3(2), normally occurs only in stationary‐phase cultures, but normal growth‐phase‐dependent production in liquid culture conditions appears to be mediated at the transcriptional level through activation of the actII‐ORF4 promoter. Expand
Regulation of the Streptomyces coelicolor Calcium-Dependent Antibiotic by absA, Encoding a Cluster-Linked Two-Component System
TLDR
This paper has identified numerous transcriptional start sites within the CDA cluster and shown that the original antibiotic-negative mutants used to identify absA exhibit a stronger negative regulation of promoters upstream of the proposed CDA biosynthetic genes than of promoters in the clusters responsible for production of actinorhodin and undecylprodigiosin. Expand
afsB stimulates transcription of the actinorhodin biosynthetic pathway in Streptomyces coelicolor A3(2) and Streptomyces lividans
TLDR
Data show that afsB exerts its positive regulatory effect by means of transcriptional stimulation of its target genes under conditions in which they are normally silent in this strain, leading to production of actinorhodin in large quantity. Expand
A microbial hormone, A-factor, as a master switch for morphological differentiation and secondary metabolism in Streptomyces griseus.
  • S. Horinouchi
  • Medicine
  • Frontiers in bioscience : a journal and virtual library
  • 2002
TLDR
Accumulating evidence has shown that a pair of genes encoding a probable gamma-butyrolactone biosynthesis enzyme and its specific receptor is contained in a number of biosynthetic gene clusters for secondary metabolites in various Streptomyces species and controls the biosynthesis of the respective metabolites by activating the pathway-specific regulatory genes. Expand
Global negative regulation of Streptomyces coelicolor antibiotic synthesis mediated by an absA-encoded putative signal transduction system
TLDR
It is proposed that the absA locus encodes a signal transduction mechanism that negatively regulates synthesis of the multiple antibiotics produced by S. coelicolor. Expand
Induction of ppGpp synthesis in Streptomyces coelicolor A3(2) grown under conditions of nutritional sufficiency elicits actII‐ORF4 transcription and actinorhodin biosynthesis
TLDR
This work provides the most compelling evidence yet for the activation of an antibiotic biosynthetic pathway by the stringent factor ppGpp. Expand
The cutRS signal transduction system of Streptomyces lividans represses the biosynthesis of the polyketide antibiotic actinorhodin.
TLDR
The nucleotide sequence of a two-component signal transduction operon (cutRS) of Streptomyces lividans TK64 was elucidated and it was shown that mutants exhibited accelerated and increased production of the polyketide antibiotic, actinorhodin, which could be reversed by introduction of cutR on a plasmid. Expand
Transcriptional regulation of the redD transcriptional activator gene accounts for growth‐phase‐dependent production of the antibiotic undecylprodigiosin in Streptomyces coelicolor A3(2)
TLDR
Transcription of redD, the activator gene required for production of the red‐pigmented antibiotic undecylprodigiosin by Streptomyces coelicolor A3(2), showed a dramatic increase during the transition from exponential to stationary phase, which appeared to be mediated entirely through the redD promoter, which shows limited similarity to the consensus sequence for the major class of eubacterial promoters. Expand
Genetic and transcriptional analysis of absA, an antibiotic gene cluster‐linked two‐component system that regulates multiple antibiotics in Streptomyces coelicolor
TLDR
A genetic analysis shows that the phosphorylated form of the AbsA2 response regulator (phospho‐AbsA2), generated by the cognate AbsA1 sensor histidine kinase, is required for normal growth phase regulation of antibiotic synthesis. Expand
Phosphate Control of the Biosynthesis of Antibiotics and Other Secondary Metabolites Is Mediated by the PhoR-PhoP System: an Unfinished Story
  • J. Martín
  • Medicine, Biology
  • Journal of bacteriology
  • 2004
TLDR
Directed phoR-phoP gene disruption will be very useful for the construction of tailored phosphate-deregulated strains overproducing valuable secondary metabolites. Expand
...
1
2
3
4
5
...