Transcriptional activation of the aldehyde reductase YqhD by YqhC and its implication in glyoxal metabolism of Escherichia coli K-12.

@article{Lee2010TranscriptionalAO,
  title={Transcriptional activation of the aldehyde reductase YqhD by YqhC and its implication in glyoxal metabolism of Escherichia coli K-12.},
  author={Changhan Lee and Insook Kim and Junghoon Lee and K Lee and Bumchan Min and Chankyu Park},
  journal={Journal of bacteriology},
  year={2010},
  volume={192 16},
  pages={4205-14}
}
The reactive alpha-oxoaldehydes such as glyoxal (GO) and methylglyoxal (MG) are generated in vivo from sugars through oxidative stress. GO and MG are believed to be removed from cells by glutathione-dependent glyoxalases and other aldehyde reductases. We isolated a number of GO-resistant (GO(r)) mutants from Escherichia coli strain MG1655 on LB plates containing 10 mM GO. By tagging the mutations with the transposon TnphoA-132 and determining their cotransductional linkages, we were able to… CONTINUE READING