Transcriptional activation of E2F1 gene expression by 17beta-estradiol in MCF-7 cells is regulated by NF-Y-Sp1/estrogen receptor interactions.

@article{Wang1999TranscriptionalAO,
  title={Transcriptional activation of E2F1 gene expression by 17beta-estradiol in MCF-7 cells is regulated by NF-Y-Sp1/estrogen receptor interactions.},
  author={Weihuang Wang and Lanlan Dong and Brad Saville and Stephen Safe},
  journal={Molecular endocrinology},
  year={1999},
  volume={13 8},
  pages={1373-87}
}
17beta-Estradiol (E2) stimulated proliferation and DNA synthesis in MCF-7 human breast cancer cells, and this was accompanied by induction of E2F1 mRNA and protein levels. Analysis of the E2F1 gene promoter showed that the -146 to -54 region was required for E2-responsiveness in transient transfection assays, and subsequent deletion/mutation analysis showed… CONTINUE READING