Transcriptional Regulation of Streptomyces coelicolorPathway-Specific Antibiotic Regulators by the absA andabsB Loci

@article{Aceti1998TranscriptionalRO,
  title={Transcriptional Regulation of Streptomyces coelicolorPathway-Specific Antibiotic Regulators by the absA andabsB Loci},
  author={David J. Aceti and Wendy C. Champness},
  journal={Journal of Bacteriology},
  year={1998},
  volume={180},
  pages={3100 - 3106}
}
ABSTRACT The four antibiotics produced by Streptomyces coelicolor are all affected by mutations in the absAand absB loci. The absA locus encodes a putative two-component signal transduction system, and theabsB locus encodes a homolog of Escherichia coli RNase III. We assessed whether these loci control synthesis of the antibiotics actinorhodin and undecylprodigiosin by regulating transcript abundance from the biosynthetic and regulatory genes specific for each antibiotic. Strains that were Abs… Expand
Genome-wide analysis of the role of the antibiotic biosynthesis regulator AbsA2 in Streptomyces coelicolor A3(2)
TLDR
The analysis of a ΔabsA2 deletion strain, which exhibits the classic precocious antibiotic hyper-production phenotype, and its complementation by an N-terminal triple-FLAG-tagged version of AbsA2 sheds light on the complex mechanism of regulation of antibiotic biosynthesis in Streptomyces coelicolor. Expand
Genome-wide analysis of the role of the antibiotic biosynthesis regulator AbsA2 in Streptomyces coelicolor A3(2)
TLDR
The analysis of a ΔabsA2 deletion strain, which exhibits the classic precocious antibiotic hyper-production phenotype, and its complementation by an N-terminal triple-FLAG-tagged version of AbsA2 sheds light on the complex mechanism of regulation of antibiotic biosynthesis in Streptomyces coelicolor. Expand
A Streptomyces coelicolor Antibiotic Regulatory Gene, absB, Encodes an RNase III Homolog
TLDR
This study isolated absB-complementing DNA and shows that it encodes the S. coelicolor homolog of RNase III (rnc), indicating that the absB mutant global defect in antibiotic synthesis is due to a deficiency inRNase III. Expand
Phosphorylated AbsA2 Negatively Regulates Antibiotic Production in Streptomyces coelicolor through Interactions with Pathway-Specific Regulatory Gene Promoters
TLDR
The data suggest that AbsA2 approximately P inhibits antibiotic production by directly interfering with the expression of pathway-specific regulators of antibiotic biosynthetic gene clusters. Expand
Regulation of the Streptomyces coelicolor Calcium-Dependent Antibiotic by absA, Encoding a Cluster-Linked Two-Component System
TLDR
This paper has identified numerous transcriptional start sites within the CDA cluster and shown that the original antibiotic-negative mutants used to identify absA exhibit a stronger negative regulation of promoters upstream of the proposed CDA biosynthetic genes than of promoters in the clusters responsible for production of actinorhodin and undecylprodigiosin. Expand
Genetic and transcriptional analysis of absA, an antibiotic gene cluster‐linked two‐component system that regulates multiple antibiotics in Streptomyces coelicolor
TLDR
A genetic analysis shows that the phosphorylated form of the AbsA2 response regulator (phospho‐AbsA2), generated by the cognate AbsA1 sensor histidine kinase, is required for normal growth phase regulation of antibiotic synthesis. Expand
Repression of Antibiotic Production and Sporulation in Streptomyces coelicolor by Overexpression of a TetR Family Transcriptional Regulator
TLDR
The direct and functional interaction of SCO3201 with the promoter region of scbA, a gene under the positive control of the TetR-like regulator, ScbR, was indeed demonstrated by in vitro as well as in vivo approaches. Expand
Genetic suppression analysis of non-antibiotic-producing mutants of the Streptomyces coelicolor absA locus.
The absA locus in Streptomyces coelicolor A3(2) was identified because mutations in it uncoupled sporulation from antibiotic synthesis: absA mutants failed to produce any of the four antibioticsExpand
Regulation of a Novel Gene Cluster Involved in Secondary Metabolite Production in Streptomyces coelicolor
TLDR
Evidence is provided for the contribution of a novel genetic locus to antibiotic production in Streptomyces coelicolor by overexpression of a gene cluster comprising four protein-encoding genes (abeABCD) and an antisense RNA-encoded gene (α-abeA). Expand
Characterization of rrdA, a TetR Family Protein Gene Involved in the Regulation of Secondary Metabolism in Streptomyces coelicolor
TLDR
An in vivo transposition system was used to identify novel regulators that influence Red production in Streptomyces coelicolor M145 and showed that RrdA negatively regulated Red production by controlling redD mRNA abundance, while no change was observed at the transcript level of the Act-specific activator gene, actII-orf4. Expand
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TLDR
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