Transcriptional Regulation of Mouse μ Opioid Receptor Gene: Sp3 Isoforms (M1, M2) Function as Repressors in Neuronal Cells to Regulate the μ Opioid Receptor Gene

@article{Choi2005TranscriptionalRO,
  title={Transcriptional Regulation of Mouse $\mu$ Opioid Receptor Gene: Sp3 Isoforms (M1, M2) Function as Repressors in Neuronal Cells to Regulate the $\mu$ Opioid Receptor Gene},
  author={Hack Sun Choi and Cheol Kyu Hwang and Chun Sung Kim and Kyu Young Song and Ping-Yee Law and Li-Na Wei and Horace H. Loh},
  journal={Molecular Pharmacology},
  year={2005},
  volume={67},
  pages={1674 - 1683}
}
The 5′-flanking region of the mouse μ opioid receptor (MOR) gene has two promoters, referred to as distal and proximal. MOR mRNA is predominantly initiated by the proximal promoter. Previously, several important cis-elements and trans-factors have been shown to play a functional role in the proximal promoter of the MOR gene. In this study, we defined another functional, negative regulatory element located in the –219- to –189-base pair (translational start site designed as +1) region of the… 

Figures from this paper

The role of transcription factors Sp1 and YY1 in proximal promoter region in initiation of transcription of the mu opioid receptor gene in human lymphocytes

It is concluded that the cooperative interaction of Sp1 and YY1 transcription factors is the critical event triggering the initiation of transcription of the MOR gene in lymphocytes, and this finding will be helpful to understand the pharmacological effect of morphine on lymphocytes.

Differential regulation of mouse and human Mu opioid receptor gene depends on the single stranded DNA structure of its promoter and α-complex protein 1.

The current study demonstrates that α-CP1 functions as a transcriptional activator in the mMOR gene, but does not function in the hMOR gene due to species-specificStructural differences in human and mouse MOR gene expression are based on α- CP1 and the ssDNA structure of the MOR promoter.

Up-Regulation of the μ-Opioid Receptor Gene Is Mediated through Chromatin Remodeling and Transcriptional Factors in Differentiated Neuronal Cells

The results suggest that during neuronal differentiation, MeCP2 and DNA methylation mediate remodeling of the MOR promoter by chromatin remodeling factors (Brg1 and BAF155) from a compacted state to a conformation allowing access for transcriptional factors.

Post‐transcriptional regulation of mouse μ opioid receptor (MOR1) via its 3′ untranslated region: a role for microRNA23b

This is the first study reporting a translationally repressive role for the MOR1 3′‐UTR, and proposes a mechanism in which miRNA23b blocks the association of MOR1 mRNA with polysomes, thereby arresting its translation and suppressing the production of MOR 1 protein.

A Major Species of Mouse μ-opioid Receptor mRNA and Its Promoter-Dependent Functional Polyadenylation Signal

MOR1 is verifies as the major mature MOR gene transcript that has the full capacity to produce functional MOR protein, identifies the 3′-UTR of MOR1 transcript, and uncovers functional coupling of the MOR gene promoter and its polyadenylation signal.

Transcription of the chicken Grin1 gene is regulated by the activity of SP3 and NRSF in undifferentiated cells and neurons.

A dual role of SP3 in regulating the expression of the Grin1 gene, by repressing transcription in the 5'-UTR in undifferentiated cells as well as acting as a transcription factor, increasing Grin 1 gene transcription in neurons is demonstrated.

Characterizing exons 11 and 1 promoters of the mu opioid receptor (Oprm) gene in transgenic mice

It is believed that these transgenic mice will provide a useful model for further characterizing the E11 and E1 promoter in vivo under different physiological and pathological circumstances such as chronic opioid treatment and chronic pain models.

Transcriptional regulation of mouse mu opioid receptor gene in neuronal cells by Poly(ADP-ribose) polymerase-1

It is reported that poly(ADP‐ribose) polymerase 1 (PARP‐1) binds to the double‐stranded poly(C) element essential for the MOR promoter and represses promoter activity at the transcriptional level and demonstrated for the first time a role of PARP‐ 1 as a transcriptional repressor in MOR gene regulation.

Epigenetic programming of μ-opioid receptor gene in mouse brain is regulated by MeCP2 and brg1 chromatin remodelling factor

It is concluded that MOR gene expression is epigenetically programmed in various brain regions and that MeCP2 assists the epigenetic program during DNA methylation and chromatin remodelling of the MOR promoter.

Evidence of the neuron-restrictive silencer factor (NRSF) interaction with Sp3 and its synergic repression to the mu opioid receptor (MOR) gene

The results suggest that the synergic interaction between NRSF and Sp3 is required to negatively regulate MOR gene transcription and that transcription of MOR gene would be governed by the context of available transcription factors rather than by a master regulator.

References

SHOWING 1-10 OF 40 REFERENCES

Mouse μ Opioid Receptor Distal Promoter Transcriptional Regulation by SOX Proteins*

Data indicate thatSOX proteins might contribute to the transcriptional activity of the mor gene and suggest that μ opioid receptor could mediate some of the developmental processes in which SOX proteins are included.

Transcriptional modulation of mouse mu-opioid receptor distal promoter activity by Sox18.

The results suggest that Sox18 directly and specifically stimulates mor gene expression, by trans-activating the mor DP enhancer, in a Sox18 concentration-dependent manner.

Single-stranded DNA-binding Complex Involved in Transcriptional Regulation of Mouse μ-Opioid Receptor Gene*

  • J. KoH. Loh
  • Biology, Chemistry
    The Journal of Biological Chemistry
  • 2001
The results suggest that transcription of mouse mor gene is regulated by an interplay of ss and ds DNA binding factors, interacting with an overlapping DNA (PPy/u) region, which is necessary for proximal promoter activation.

Transcriptional Regulation of Mouse μ Opioid Receptor Gene by PU.1*

The results suggest that PU.1 may be an important regulator of the MOR gene, particularly in brain and immune cells, as well as in neuroblastoma NMB cells.

Transcriptional Regulation of Mouse δ-Opioid Receptor Gene*

The distinct developmental emergence and brain regional distribution of the δ opioid receptor appear to be controlled, at least in part, by these two regulatory elements and their associated factors.

Dual Promoters of Mouse μ-Opioid Receptor Gene1

To verify the physiological importance of each promoter, the ratio of mu-receptor transcripts initiated by either promoter was examined by quantitative RT-PCR using mouse adult brain mRNA and found that receptor mRNA was predominantly initiated by the proximal promoter.

Dual promoters of mouse mu-opioid receptor gene.

To verify the physiological importance of each promoter, the ratio of mu-receptor transcripts initiated by either promoter was examined by quantitative RT-PCR using mouse adult brain mRNA and found that receptor mRNA was predominantly initiated by the proximal promoter.

Transcriptional regulation of mouse delta-opioid receptor gene by CpG methylation: involvement of Sp3 and a methyl-CpG-binding protein, MBD2, in transcriptional repression of mouse delta-opioid receptor gene in Neuro2A cells.

It is demonstrated that Sp3 and MBD2 are involved in the transcriptional repression of mDOR in Neuro2A cells through binding to the methylated CpG sites in the promoter region and may play a role in the cell type-specific expression ofmDOR.

Genomic structure analysis of promoter sequence of a mouse mu opioid receptor gene.

Comparison of the 5' flanking sequence with a transcription factor database revealed putative cis-acting regulatory elements for transcription factors affected by cAMP, as well as those involved in the action of gluco- and mineralocorticoids, cytokines, and immune-cell-specific factors.

Transcriptional Regulation of Mouse δ-Opioid Receptor Gene by CpG Methylation

It is demonstrated that Sp3 and MBD2 are involved in the transcriptional repression of mDOR in Neuro2A cells through binding to the methylated CpG sites in the promoter region and may play a role in the cell type-specific expression of m DOR.