Transcription termination factor rho: the site of bicyclomycin inhibition in Escherichia coli.

@article{Zwiefka1993TranscriptionTF,
  title={Transcription termination factor rho: the site of bicyclomycin inhibition in Escherichia coli.},
  author={Antoni Zwiefka and Harold L Kohn and William Widger},
  journal={Biochemistry},
  year={1993},
  volume={32 14},
  pages={
          3564-70
        }
}
Bicyclomycin is a novel, commercially important antibiotic. Information concerning the site of bicyclomycin inhibition in Escherichia coli has been obtained by the production of bicyclomycin resistant mutants by UV irradiation. Selection by growth in the presence of bicyclomycin of a plasmid clone library generated from a highly resistant mutant in recipient antibiotic-sensitive host cells (E. coli strain W3350) has led to the characterization of three different plasmids that confer drug… 
The Antibiotic Bicyclomycin Affects the Secondary RNA Binding Site of Escherichia coli Transcription Termination Factor Rho*
TLDR
The data suggested that the antibiotic binds to Rho, influencing the secondary RNA binding (tracking) site on Rho and slows the tracking of Rho toward the bound RNA polymerase.
Bicyclomycin sensitivity and resistance affect Rho factor-mediated transcription termination in the tna operon of Escherichia coli
TLDR
The growth-inhibiting drug bicyclomycin was shown to increase basal level expression of the tryptophanase (tna) operon and to allow growth of a tryPTophan auxotroph on indole, which demonstrates that resistance to this drug may be required by mutations at any one of three loci, two of which appear to be rho and rpoB.
The bicyclomycin sensitivities of 38 bicyclomycin-resistant mutants of transcription termination protein rho and the location of their mutations support a structural model of rho based on the F(1) ATPase.
TLDR
The bicyclomycin sensitivities of the mutants are consistent with the locations of their respective mutations in the model of Rho based on the F(1) ATPase, therefore supporting the emerging consensus model of E. coli transcription termination protein Rho structure.
Evidence for the Location of Bicyclomycin Binding to theEscherichia coli Transcription Termination Factor Rho*
TLDR
Findings provide the first structural information concerning the site of Bcm binding in Rho.
Termination Factor Rho and Its Cofactors NusA and NusG Silence Foreign DNA in E. coli
TLDR
Rho is revealed as a global regulator of gene expression that matches Escherichia coli transcription to translational needs and that genes in E. coli that are most repressed by Rho are prophages and other horizontally acquired portions of the genome.
Reproducing tna Operon Regulation in Vitro in an S-30 System
TLDR
It is shown that transcription initiation is cyclic AMP (cAMP)-dependent and is not influenced by tryptophan, and that inducer acts by preventing cleavage of TnaC peptidyl-tRNA.
Recombinant yeast and human cells as screening tools to search for antibacterial agents targeting the transcription termination factor Rho
TLDR
The design of new tools to screen for target-specific inhibitors of the bacterial Rho factor directly inside eukaryotic cells are described and it is shown that Rho factors from different bacterial pathogens can also be designed as yeast-based screening tools which can reveal subtle variations in the functional features of the proteins.
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References

SHOWING 1-10 OF 25 REFERENCES
Interaction of RNA polymerase and rho in transcription termination: coupled ATPase.
  • A. Das, C. Merril, S. Adhya
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1978
TLDR
The isolation of mutants altered in the beta subunit of RNA polymerase (a class of Rifampicin-resistant mutants), which restore gal IS2 polarity in the rho 15 strain are reported, which strongly support the notion that rho andRNA polymerase interact functionally during transcription termination.
Isolation and characterization of conditional lethal mutants of Escherichia coli defective in transcription termination factor rho.
  • A. Das, D. Court, S. Adhya
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1976
TLDR
The results suggest that rho has an essential function in the growth and normal physiology of cells and supports the model that N gene product prevents transcription termination by antagonizing rho activity.
Isolation and characterization of conditional-lethal rho mutants of Escherichia coli.
TLDR
The production of thermally unstable rho by the nitA temperature-sensitive mutant suggests that nitA is the structural gene for rho, andTemperature-sensitive nitA bacteria not only permit leftward transcription of thelambda genome at a high rate in the absence of the lambda N protein, but also allow lambda growth at low temperatures.
rho Factors from polarity suppressor mutants with defects in their RNA interactions.
TLDR
The results suggest that the defects in the rho factors isolated from strains with the polarity suppressor alleles suA1 and suA100 involve their abilities to interact with RNA at their secondary sites.
Replacement of the fip gene of Escherichia coli by an inactive gene cloned on a plasmid
TLDR
To determine whether the fip gene of Escherichia coli is required for filamentous phage assembly, the chromosomal copy of the gene was replaced with an inactive copy introduced on a plasmid and it was found that thefip gene is dispensable.
Structural modification of Escherichia coli peptidoglycan induced by bicyclomycin.
TLDR
The results show that bicyclomycin impairs the normal breakage of that interpeptidic bond, whose cleavage is needed for the normal remodeling of peptidoglycan and cell growth.
BICYCLOMYCIN, A NEW ANTIBIOTIC
TLDR
Bicyclomycin, a new antibiotic active against Gram-negative bacteria and having a unique chemical structure, was investigated for its absorption, excretion and tissue distribution and when given orally, bicyclomycin was absorbed to a certain extent in rats, but only to a limited extent in human volunteers.
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