Transcription termination and anti‐termination in E. coli

  title={Transcription termination and anti‐termination in E. coli},
  author={Evgeny Nudler and Max E. Gottesman},
  journal={Genes to Cells},
Transcription termination in Escherichia coli is controlled by many factors. The sequence of the DNA template, the structure of the transcript, and the actions of auxiliary proteins all play a role in determining the efficiency of the process. Termination is regulated and can be enhanced or suppressed by host and phage proteins. This complex reaction is rapidly yielding to biochemical and structural analysis of the interacting factors. Below we review and attempt to unify into basic principles… 
The role of E. coli Nus-Factors in transcription regulation and transcription
The structural interplay and the mechanistic role of the Nus-factors that are directly involved in processive elongation, transcription:translation coupling and termination as well as the varying effects of these proteins on transcription under the influence of additional signals are discussed.
Control of Transcription Termination and Antitermination
The regulatory molecules that are known to influence the elongation/termination decision by RNA polymerase (RNAP) are described, with the emphasis on the most recent findings and on the mechanism of ‘‘active’’ regulators whose actions are not limited to changes in RNA folding.
Transcription termination by nuclear RNA polymerases.
The present state of knowledge on how each of the three RNA polymerases terminates and how mechanisms are conserved, or vary, from yeast to human is described.
Suppression of Factor-Dependent TranscriptionTermination by AntiterminatorRNA
Nascent transcripts of the phage HK022 put sites modify the transcription elongation complex so that it terminates less efficiently at intrinsic transcription terminators and accelerates through pause sites, suggesting that put inhibits a step that is common to termination at the different types of terminator.
Regulation of Transcription Elongation and Termination
Why transcription elongation complexes pause at certain template sites and how auxiliary host and phage transcription factors affect elongation and termination in E. coli are discussed.
Mechanism of Action of Bacterial Transcription Terminator Rho
This review includes the biochemistry and the structural analyses of the Rho, its mechanism of action, its regulation by other cellular factors and briefly cellular events controlled by this protein.
sRNA-Mediated Control of Transcription Termination in E. coli
Loading Rho to Terminate Transcription


Mechanism of intrinsic transcription termination and antitermination.
A transcription antiterminator inhibits this activity of oligonucleotides and therefore protects the elongation complex from destabilizing attacks on the emerging transcript.
The nus mutations affect transcription termination in Escherichia coli
Observations suggest that the nusA and nusB gene products may act as termination factors analgous to rho protein in Escherichia coli and enhance transcription termination atphage termination sites.
Transcription termination control in bacteria.
  • T. Henkin
  • Biology
    Current opinion in microbiology
  • 2000
Regulation of rho-dependent transcription termination by NusG is specific to the Escherichia coli elongation complex.
It is concluded that NusG modulates Rho-dependent termination by interacting specifically with the RNAP of the E. coli elongation complex to render the complex more susceptible to the termination activity of Rho.
Structural Organization of Transcription Termination Factor Rho (*)
  • J. Richardson
  • Biology, Chemistry
    The Journal of Biological Chemistry
  • 1996
The current understanding of the structure and function of transcription termination factor Rho is summarized based on indirect approaches, such as biochemical characterization of the protein, phylogenetic comparative analyses, and the functional properties of mutants with known amino acid changes.