Transcription of vaccinia virus early genes by enzymes isolated from vaccinia virions terminates downstream of a regulatory sequence.

@article{Rohrmann1986TranscriptionOV,
  title={Transcription of vaccinia virus early genes by enzymes isolated from vaccinia virions terminates downstream of a regulatory sequence.},
  author={George F. Rohrmann and L Yuen and Bernard Moss},
  journal={Cell},
  year={1986},
  volume={46 7},
  pages={1029-35}
}
We describe an in vitro transcription system in which the polyadenylated 3' ends of vaccinia virus mRNAs are formed by termination downstream of a regulatory signal. When linear DNA templates containing vaccinia early genes were incubated with soluble enzymes extracted from vaccinia virions, mature-size mRNAs were synthesized within 1 min, whereas longer… CONTINUE READING