Transcription of reference genes used for quantitative RT-PCR in Atlantic salmon is affected by viral infection


Relative quantification using RT-qPCR is a widely used method for transcription profiling. Transcript levels of target genes in fish after experimental infection is often reported without documentation of stably transcribed reference genes. We present results demonstrating that transcription of typically used reference genes in Atlantic salmon is not stable… (More)
DOI: 10.1186/1297-9716-42-8


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