Transcription of rat DNA fractions reassociated to various cOt values.


Two fractions of rat liver DNA: "intermediate" (reassociated to COt = 1 - 10(2), moleXsecX1(-1)) and "slow" (COt = 10(2) - 10(5) showed differences in template activity on transcription with E. coli RNA-polymerase. The "intermediate" fraction both in double- and single-stranded form was a better template than the "slow" one. The efficiency of transcription… (More)