Transcription of laminin gamma1 chain gene in rat mesangial cells: constitutive and inducible RNA polymerase II recruitment and chromatin states.

@article{Nelson2008TranscriptionOL,
  title={Transcription of laminin gamma1 chain gene in rat mesangial cells: constitutive and inducible RNA polymerase II recruitment and chromatin states.},
  author={Joel D. Nelson and Steve Flanagin and Yasunobu Kawata and Oleg Denisenko and Karol Bomsztyk},
  journal={American journal of physiology. Renal physiology},
  year={2008},
  volume={294 3},
  pages={F525-33}
}
The laminin gamma1 chain, a critical component of the extracellular matrix, is encoded by the 125-kb-long Lamc1 locus. We profiled RNA polymerase II (Pol II) and histone modifications along the Lamc1 locus to explore transcription of this gene in its native chromatin environment. Treatment with 12-O-tetradecanoylphorbol-13-acetate increased Lamc1 mRNA in rat mesangial cells (RMC). This increase was matched by an increase in Pol II density along the entire length of the Lamc1 locus. In contrast… CONTINUE READING