Transcription of a recombinant bunyavirus RNA template by transiently expressed bunyavirus proteins.

@article{Dunn1995TranscriptionOA,
  title={Transcription of a recombinant bunyavirus RNA template by transiently expressed bunyavirus proteins.},
  author={Ewan F Dunn and D C Pritlove and Hong Jin and Richard M. Elliott},
  journal={Virology},
  year={1995},
  volume={211 1},
  pages={133-43}
}
We describe a convenient system for analyzing bunyavirus transcription using a recombinant RNA template derived from the plasmid pBUNSCAT, which comprises a negative-sense reporter gene (chloramphenicol acetyltransferase or CAT) flanked by the exact 5' and 3' untranslated regions of the Bunyamwera virus (BUN) S RNA segment. When cells which expressed bunyavirus proteins (either by recombinant vaccinia viruses or by the vaccinia virus-T7 system) were transfected with BUNSCAT RNA, CAT activity… CONTINUE READING

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