Transcription of Vibrio parahaemolyticus T3SS1 genes is regulated by a dual regulation system consisting of the ExsACDE regulatory cascade and H-NS.

@article{Kodama2010TranscriptionOV,
  title={Transcription of Vibrio parahaemolyticus T3SS1 genes is regulated by a dual regulation system consisting of the ExsACDE regulatory cascade and H-NS.},
  author={T. Kodama and C. Yamazaki and Kwon-sam Park and Y. Akeda and T. Iida and T. Honda},
  journal={FEMS microbiology letters},
  year={2010},
  volume={311 1},
  pages={
          10-7
        }
}
Vibrio parahaemolyticus, one of the human pathogenic vibrios, causes gastroenteritis, wound infections and septicemia. Genomic sequencing of this organism revealed that it has two distinct type III secretion systems (T3SS1 and T3SS2). T3SS1 plays a significant role in lethal activity in a murine infection model. It was reported that expression of the T3SS1 gene is controlled by a positive regulator, ExsA, and a negative regulator, ExsD, which share a degree of sequence similarity with… Expand
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Transcription of exsD is repressed directly by H-NS in Vibrio parahaemolyticus.
TLDR
All three genes in the ExsA-ExsC- exsD regulatory system of T3SS1 are directly repressed by H-NS in V. parahaemolyticus, a leading cause of seafood-associated diarrhea and gastroenteritis. Expand
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References

SHOWING 1-10 OF 39 REFERENCES
Two Regulators of Vibrio parahaemolyticus Play Important Roles in Enterotoxicity by Controlling the Expression of Genes in the Vp-PAI Region
TLDR
It is reported that two novel ToxR-like transcriptional regulatory proteins (VtrA and VtrB) regulate the expression of the genes encoded within the Vp-PAI region, including those for TDH and T3SS2-related proteins. Expand
Functional Characterization of Two Type III Secretion Systems of Vibrio parahaemolyticus
TLDR
Results provide evidence that V. parahaemolyticus TTSSs function as secretion systems and may have a role in the pathogenicity of the organism, and is the first report of functional T TSSs in Vibrio species. Expand
Identification of Two Translocon Proteins of Vibrio parahaemolyticus Type III Secretion System 2
TLDR
Functional analysis of VopB2 and VopD2 showed that they are essential for T3SS2-dependent cytotoxicity, for the translocation of one of the T3 SS2 effector proteins (VopT), and for the contact-dependent activity of pore formation in infected cells in vitro. Expand
Identification and characterization of VopT, a novel ADP‐ribosyltransferase effector protein secreted via the Vibrio parahaemolyticus type III secretion system 2
TLDR
It is reported that T3SS2 induces a cytotoxic effect in Caco‐2 and HCT‐8 cells and it is revealed that VPA1327 (vopT), a gene encoded within the proximity of T3 SS2, is partly responsible for this cytotoxicity effect, indicating that VopT is a novel ADPRT effector secreted via V. parahaemolyticus T3ss. Expand
Contribution of Vibrio parahaemolyticus Virulence Factors to Cytotoxicity, Enterotoxicity, and Lethality in Mice
TLDR
Interestingly, it is found that both T3SS1 and TDH played a significant role in lethal activity in a murine infection model, which provides new indications that these virulence factors contribute to and orchestrate each distinct aspect of the pathogenicity of V. parahaemolyticus. Expand
Identification of Proteins Secreted via Vibrio parahaemolyticus Type III Secretion System 1
TLDR
Flow cytometry analysis using fluorescence-activated cell sorting with fluorescein isothiocyanate-labeled annexin V demonstrated that the TTSS1-dependent cell death was by apoptosis, and cytotoxicity to HeLa cells was related to one of the newly identified secreted proteins encoded by VP1680. Expand
Identification and characterization of a type III secretion-associated chaperone in the type III secretion system 1 of Vibrio parahaemolyticus.
TLDR
It is concluded that there is a T3S-associated chaperone, VecA, for a cytotoxic T3SS1-dependent effector, VepA, and this findings will provide a strategy to clarify how the T3 SS1 of V. parahaemolyticus secretes its specific effectors. Expand
Type III secretion system 1 genes in Vibrio parahaemolyticus are positively regulated by ExsA and negatively regulated by ExsD
TLDR
A gel mobility shift assay demonstrated that purified ExsA protein binds a novel motif in the upstream region of vp1668 and vp 1687, indicating that Exs a interacts directly with the promoter sequences of T3SS1 genes. Expand
Vibrio parahaemolyticus Inhibition of Rho Family GTPase Activation Requires a Functional Chromosome I Type III Secretion System
TLDR
The ability to inhibit Rho family GTPases independently of the CII-T3SS and the hemolytic toxins may provide insight into the mechanisms of virulence used by strains lacking these virulence factors. Expand
The exoenzyme S regulon of Pseudomonas aeruginosa
  • D. Frank
  • Biology, Medicine
  • Molecular microbiology
  • 1997
TLDR
Using the expression of ExoS as a model, a series of genes that are important for regulation, secretion and, perhaps, intoxication of eukaryotic cells have been identified and it will be important to understand how the exoenzyme S regulon contributes to pathogenesis and whether these factors could serve as potential therapeutic targets. Expand
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