Transcription of RNA templates by T7 RNA polymerase.

  title={Transcription of RNA templates by T7 RNA polymerase.},
  author={N Arnaud-Barbe and V Cheynet-Sauvion and Guy Oriol and Bernard Mandrand and François Mallet},
  journal={Nucleic acids research},
  volume={26 15},
Although highly specialized, T7 RNA polymerase seems to possess a large range of DNA- and RNA-dependent properties. To study such flexibility, we determined the ability of T7 RNA polymerase to transcribe chimeric DNA-RNA and RNA templates following initiation at a double stranded DNA promoter. We have found that T7 RNA polymerase is able to initiate on RNA templates, was processive, and was able to use templates containing RNA-RNA duplexes under standard transcription conditions. Implications… CONTINUE READING