Transcription mapping of the Escherichia coli chromosome by electron microscopy.

  title={Transcription mapping of the Escherichia coli chromosome by electron microscopy.},
  author={Sarah L. French and O. A. Hurricane P. L. Miller},
  journal={Journal of bacteriology},
  volume={171 8},
The distinctive double Christmas tree morphology of rRNA operons as visualized by electron microscopy makes them easy to recognize in chromatin spreads from Escherichia coli. On the basis of the pattern of nascent transcripts on nearby transcription units and the relative distances of the operons from one another and the replication origin, we are now able to specifically identify five of the seven rRNA operons in E. coli. The use of rRNA operons as markers of both position and distance has… CONTINUE READING

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