Transcription in yeast activated by a putative amphipathic alpha helix linked to a DNA binding unit.

@article{Giniger1987TranscriptionIY,
  title={Transcription in yeast activated by a putative amphipathic alpha helix linked to a DNA binding unit.},
  author={Edward Giniger and Mark Ptashne},
  journal={Nature},
  year={1987},
  volume={330 6149},
  pages={670-2}
}
Gene activation by a DNA-binding regulatory protein in yeast requires the protein to have two components: one to recognize a specific DNA sequence and a second, the 'activating region', to interact with a general transcription factor or perhaps with RNA polymerase. The activating regions that have been characterized are acidic, and mutational analysis of one indicates that this acidity is important for activity. Here we report the design of an artificial protein bearing a novel 15-amino acid… CONTINUE READING

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