Transcription in vitro promoted by the Agrobacterium VirG protein.

@article{Endoh1993TranscriptionIV,
  title={Transcription in vitro promoted by the Agrobacterium VirG protein.},
  author={Hideki Endoh and Takashi Aoyama and Atsuhiro Oka},
  journal={FEBS letters},
  year={1993},
  volume={334 3},
  pages={277-80}
}
Expression of the virulence genes (vir) on the hairy-root-inducing plasmid pRiA4 is induced by plant signals in Agrobacterium cells through a two-component regulatory system, the VirA-VirG system. We constructed an in vitro transcription system that consisted of the purified VirG protein and the Agrobacterium RNA polymerase holoenzyme. Both versions of VirG… CONTINUE READING