Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics

  title={Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics},
  author={Toyotaka Ishibashi and Manchuta Dangkulwanich and Yves Coello and Troy A. Lionberger and Lucyna Lubkowska and Alfred S. Ponticelli and Mikhail Kashlev and Carlos J. Bustamante},
  journal={Proceedings of the National Academy of Sciences},
  pages={3419 - 3424}
Significance Regulation of gene expression controls fundamental cellular processes, such as growth and differentiation. Gene expression begins at transcription, in which the eukaryotic RNA polymerase (Pol II) synthesizes the precursors of mRNA during the elongation phase. The speed and fidelity of the process are regulated by many transcription elongation factors, including transcription factors IIS (TFIIS) and IIF (TFIIF), which directly interact with the enzyme. Using a single-molecule… 

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