Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics

@article{Ishibashi2014TranscriptionFI,
  title={Transcription factors IIS and IIF enhance transcription efficiency by differentially modifying RNA polymerase pausing dynamics},
  author={Toyotaka Ishibashi and Manchuta Dangkulwanich and Yves Coello and Troy A. Lionberger and Lucyna Lubkowska and Alfred S. Ponticelli and Mikhail Kashlev and Carlos J. Bustamante},
  journal={Proceedings of the National Academy of Sciences},
  year={2014},
  volume={111},
  pages={3419 - 3424}
}
Significance Regulation of gene expression controls fundamental cellular processes, such as growth and differentiation. Gene expression begins at transcription, in which the eukaryotic RNA polymerase (Pol II) synthesizes the precursors of mRNA during the elongation phase. The speed and fidelity of the process are regulated by many transcription elongation factors, including transcription factors IIS (TFIIS) and IIF (TFIIF), which directly interact with the enzyme. Using a single-molecule… 

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References

SHOWING 1-10 OF 41 REFERENCES

Combinatorial Control of Human RNA Polymerase II (RNAP II) Pausing and Transcript Cleavage by Transcription Factor IIF, Hepatitis δ Antigen, and Stimulatory Factor II*

In the presence of HDAg and SII, pausing is observed without stimulation of transcript cleavage, indicating that the EC can pause without backtracking beyond the pre-translocated state.

Efficient and Rapid Nucleosome Traversal by RNA Polymerase II Depends on a Combination of Transcript Elongation Factors*

It is found that nucleosomes with a Sin mutant histone are traversed to the same extent and at nearly the same rate as equivalent pure DNA templates if both TFIIS and TFIIF are present, indicating that the nucleosome is not necessarily an insurmountable barrier to transcript elongation by pol II.

Genomic location of the human RNA polymerase II general machinery: evidence for a role of TFIIF and Rpb7 at both early and late stages of transcription.

The results provide for the first time a general picture of GTF function during the RNAPII transcription reaction in live mammalian cells and show that TFIIF and Rpb7 are involved in both early and late transcriptional stages.

Structural visualization of key steps in human transcription initiation

These structural analyses provide pseudo-atomic models at various stages of transcription initiation that illuminate critical molecular interactions, including how TFIIF engages Pol II and promoter DNA to stabilize both the closed pre-initiation complex and the open-promoter complex, and to regulate start--initiation complexes.

Elongation by RNA polymerase II: the short and long of it.

The many factors that regulate the elongation stage of transcription are discussed, including the classical elongation factors that modulate the activity of RNAP II, and the more recently identified factors that facilitate elongation on chromatin templates.

Stimulation of RNA polymerase II transcript cleavage activity contributes to maintain transcriptional fidelity in yeast

Investigation of the role of nascent transcript cleavage stimulation activity on the maintenance of transcriptional fidelity in yeast suggests that both S‐II and Rpb9 maintain transcriptionalidelity by stimulating the cleavage activity intrinsic to RNA polymerase II.