Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II

@article{Goodrich1994TranscriptionFI,
  title={Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II},
  author={James A. Goodrich and Robert Tjian},
  journal={Cell},
  year={1994},
  volume={77},
  pages={145-156}
}
Using a defined RNA polymerase II (pol II) transcription system, we have investigated the roles of basal factors at discrete stages during the transcription cycle (e.g., initiation, promoter clearance, and transcript elongation). Abortive initiation assays revealed that TATA-binding protein, transcription factors TFIIB and TFIIF, and pol II were necessary and sufficient to form functional initiation complexes on both linear and supercoiled templates. By contrast, TFIIE, TFIIH, and ATP… Expand
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References

SHOWING 1-10 OF 48 REFERENCES
Factors involved in specific transcription by mammalian RNA polymerase II. Factors IIE and IIF independently interact with RNA polymerase II.
TLDR
Interestingly TFIIF was absolutely required for the formation of a preinitiation complex; however, it also affected the elongation phase of the transcription cycle, and was required for efficient elongation. Expand
DNA topology and a minimal set of basal factors for transcription by RNA polymerase II
TLDR
The more negatively supercoiled the IgH template DNA was, the more active the transcription and the free energy of supercoiling promotes the formation of an open complex for initiation of transcription by the minimal set of transcription factors. Expand
Five intermediate complexes in transcription initiation by RNA polymerase II
TLDR
A native gel electrophoresis DNA binding assay was used to resolve complexes formed on the adenovirus Major Late Promoter by general transcription factors and RNA polymerase II, generating new complexes that contained accurately initiated transcripts associated with the transcription machinery and the template DNA. Expand
Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF
TLDR
The RAP74 protein from purified HeLa cells is partially sequenced, cloned its complementary DNA and shown that its translation product can interact with RAP30 in vitro as well as in vivo, and the cDNA predicts an amino-acid sequence that lacks obvious DNA or RNA helicase motifs. Expand
Identification of a minimal set of proteins that is sufficient for accurate initiation of transcription by RNA polymerase II.
TLDR
It appears that RNA polymerase II is able to initiate transcription subsequent to assembly of the DBPolF30 complex, which is a minitranscription complex that represents the central core of the RNA polymer enzyme II transcriptional machinery. Expand
Energy requirement for specific transcription initiation by the human RNA polymerase II system.
TLDR
The particular transcription factor that requires ATP (or dATP) hydrolysis for its function must act prior to, or concomitant with, formation of the first few phosphodiester linkages by the RNA polymerase II. Expand
RAP30/74 (transcription factor IIF) is required for promoter escape by RNA polymerase II.
TLDR
RAP30 is an initiation factor, RAP74 is not required for ATP hydrolysis in initiation, which precedes phosphodiester bond formation, and RAP 74 is not necessary for template strand separation. Expand
A common ATP requirement for open complex formation and transcription at promoters containing initiator or TATA elements.
TLDR
The results suggest that TATA-dependent and initiator-dependent preinitiation complex assembly pathways converge, prior to a step in which the strands are rapidly opened in the presence of ATP, to form analogous open complexes. Expand
Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex.
TLDR
It is found that yeast and human TFIID yielded DAB complexes with different stabilities, and anti-TFIIB antibodies and reagents that affect the stability of a transcription-competent complex are found. Expand
Mechanism of RNA polymerase II-specific initiation of transcription in vitro: ATP requirement and uncapped runoff transcripts
TLDR
It is concluded that the availability of the beta-gamma bond of ATP is an indispensable requirement for faithful and specific in vitro initiation only by RNA polymerase II in the whole cell extract. Expand
...
1
2
3
4
5
...