Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II

  title={Transcription factors IIE and IIH and ATP hydrolysis direct promoter clearance by RNA polymerase II},
  author={James A. Goodrich and Robert Tjian},

The General Transcription Machinery and Preinitiation Complex Formation

The properties of pol II and each GTF are discussed in relation to PIC assembly, which occurs prior to the formation of the first phosphodiester bond in transcription initiation, a critical and often rate-limiting step in transcriptional regulation.

The Dynamic Association of RNA Polymerase II with Initiation, Elongation, and RNA Processing Factors during the Transcription Cycle

The dynamic nature of the RNAPII holoenzyme and the ever-increasing number ofRNAPII-associated factors being identified that regulate transcription at the levels of initiation, elongation, and processing will be discussed in this chapter.

Direct Modulation of RNA Polymerase Core Functions by Basal Transcription Factors

TFB mutations are complemented by TFE, thereby demonstrating that both factors act synergistically during transcription initiation, and an additional function of TFE is to dynamically alter the nucleic acid-binding properties of RNAP by stabilizing the initiation complex and destabilizing elongation complexes.

Recycling of the general transcription factors during RNA polymerase II transcription.

It is demonstrated that all of the basal factors coexist in mature initiation complexes but that following nucleotide addition, this complex becomes disrupted.

Ctk1 promotes dissociation of basal transcription factors from elongating RNA polymerase II

The results suggest that Ctk1 is necessary for the release of RNApII from basal transcription factors, and this function of Cks1 is independent of its kinase activity, suggesting a structural function of the protein.

A Role for ATP and TFIIH in Activation of the RNA Polymerase II Preinitiation Complex Prior to Transcription Initiation (*)

The findings indicate that ATP, TFIIE, and TFIIH can have a profound effect on the efficiency of transcription initiation, and it is demonstrated that the activated initiation complex is unstable and decays rapidly to an inactive state in the presence of the inhibitor ATPS.

TFIIE orchestrates the recruitment of the TFIIH kinase module at promoter before release during transcription

Evidence is provided that theTFIIEα and TFIIEβ subunits anchor the TFIIH kinase module within the preinitiation complex before their release during transcription, which affects early transcriptional events associated with the developmental disorder, trichothiodystrophy.

Structural visualization of key steps in human transcription initiation

These structural analyses provide pseudo-atomic models at various stages of transcription initiation that illuminate critical molecular interactions, including how TFIIF engages Pol II and promoter DNA to stabilize both the closed pre-initiation complex and the open-promoter complex, and to regulate start--initiation complexes.

Transcription Start Site Scanning and the Requirement for ATP during Transcription Initiation by RNA Polymerase II*

Results show that Pol II transcription plays an important role in TSS selection but is not required for the scanning reaction, and that transcription in the purified yeast basal system is largely ATP-independent despite a requirement for the TFIIH DNA translocase subunit Ssl2.



Characterization of cDNA for the large subunit of the transcription initiation factor TFIIF

The RAP74 protein from purified HeLa cells is partially sequenced, cloned its complementary DNA and shown that its translation product can interact with RAP30 in vitro as well as in vivo, and the cDNA predicts an amino-acid sequence that lacks obvious DNA or RNA helicase motifs.

Identification of a minimal set of proteins that is sufficient for accurate initiation of transcription by RNA polymerase II.

It appears that RNA polymerase II is able to initiate transcription subsequent to assembly of the DBPolF30 complex, which is a minitranscription complex that represents the central core of the RNA polymer enzyme II transcriptional machinery.

RAP30/74 (transcription factor IIF) is required for promoter escape by RNA polymerase II.

RAP30 is an initiation factor, RAP74 is not required for ATP hydrolysis in initiation, which precedes phosphodiester bond formation, and RAP 74 is not necessary for template strand separation.

Factors involved in specific transcription by mammalian RNA polymerase II: role of transcription factors IIA, IID, and IIB during formation of a transcription-competent complex

It is found that yeast and human TFIID yielded DAB complexes with different stabilities, and anti-TFIIB antibodies and reagents that affect the stability of a transcription-competent complex are found.