Transcription factor IIA derepresses TATA-binding protein (TBP)-associated factor inhibition of TBP-DNA binding.

@article{Ozer1998TranscriptionFI,
  title={Transcription factor IIA derepresses TATA-binding protein (TBP)-associated factor inhibition of TBP-DNA binding.},
  author={Josef Ozer and Katherine Mitsouras and D Zerby and Michael Carey and Paul M. Lieberman},
  journal={The Journal of biological chemistry},
  year={1998},
  volume={273 23},
  pages={14293-300}
}
The interaction of the general transcription factor (TF) IIA with TFIID is required for transcription activation in vitro. TFIID consists of the TATA-binding protein (TBP) and TBP associated factors (TAFIIs). TFIIA binds directly to TBP and stabilizes its interaction with TATA-containing DNA. In this work, we present evidence that TAFIIs inhibit TBP-DNA and TBP-TFIIA binding, and that TFIIA stimulates transcription, in part, by overcoming this TAFII-mediated inhibition of TBP-DNA binding. TFIIA… CONTINUE READING